Acta Vet. Brno 2006, 75: 235-240

cpn60 Gene-Based Multiplex-PCR Assay for Simultaneous Identification of Streptococcal Species

A. Dmitriev1, M. Bhide2, I. Mikula2

1Institute of Experimental Medicine, Saint-Petersburg, Russia
2University of Veterinary Medicine, Košice, and Institute of Neuroimmunology, Bratislava, Slovakia

Received October 18, 2005
Accepted April 13, 2006

The cpn60 genes of Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis were sequenced and a certain polymorphism of cpn60 genes was revealed. Presumable species-specific pairs of primers were designed and their specificity was confirmed by PCR. Based on these data, the cpn60 gene-based multiplex-PCR assay was developed. It was found to be effective for simultaneous identification of S. agalactiae, S. dysgalactiae and S. uberis strains.