Acta Vet. Brno 2006, 75: 393-401

Morphological Image of Fresh and Cryopreserved Dog Semen Evaluated by the Strict Analysis of Sperm Morphology Method, Using Sperm Quality Analyzer (SQA IIc) Evaluation

P. Přinosilová1, A. Vinkler2, V. Věžník1

1Veterinary Research Institute, Brno, Czech Republic
2University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic

Received October 4, 2005
Accepted April 13, 2006

Thirty fresh ejaculates from 15 dogs were cryopreserved in Tris-fructose-citric acid-egg-yolk extender with a glycerol content of 6%. Semen samples were examined by the methods of routine sperm analysis and by the SQA IIc device. The routine semen examination focused on the evaluation of parameters determining the quality of sperm membranes. The significance of monitoring semen quality in the course of the short-term survival test for predicting dog semen quality after thawing was assessed. Relevance of the assessment of sperm morphology, and above all the percentage of sperm with membrane changes in the acrosomal region was documented. The fact that the SQA device analyses semen quality by evaluating the mass of moving cells was confirmed. The results provided by the SQA IIc device appear insufficient for the needs of deeper dog semen analysis, especially morphology assessment.