Acta Vet. Brno 2007, 76: 363-370

Protective Effects of Flavonoid Pomiferin on Heart Ischemia-Reperfusion

J. Nečas1, L. Bartošíková1, T. Florian2, J. Klusáková3, V. Suchý2, E. Janoštíková2, T. Bartošík3, Em. B. El Naggar2

1Department of Physiology, Faculty of Medicine and Dentistry, Palacký University Olomouc
2Pharmaceutical Faculty, University of Veterinary and Pharmaceutical Sciences Brno
3St. Anne Teaching Hospital Brno

Received January 23, 2006
Accepted June 5, 2007

The objective of the present 15-day study was to evaluate the cardioprotective potential of flavonoid pomiferin isolated from the infructences of Maclura pomifera, Moraceae, against ischemia-reperfusion induced injury in rat hearts as a model of antioxidant-based composite therapy. Studies were performed with isolated, modifi ed Langendorff-perfused rat hearts and ischemia of heart was initiated by stopping the coronary flow for 30 min, followed by 60 min of reperfusion (14 ml min-1). Wistar rats were divided into three groups. The treated group received pomiferin (5 mg/kg/day in 0.5% Avicel); the placebo group received only 0.5% Avicel; the intact group was left without any applications. Biochemical indicators of oxidative damage, lipid peroxidation product malondialdehyde, antioxidant enzymes (superoxide dismutase, glutathione peroxidase, total antioxidant activity in serum and myocardium has been evaluated. We also examined the effect of pomiferin on cardiac function (left ventricular end-diastolic pressure, left ventricular pressure, peak positive +dP/dt (rate of pressure development) after ischemia and reperfusion. Our results demonstrate that pomiferin attenuates the myocardial dysfunction provoked by ischemiareperfusion. This was confirmed by the increase in both the antioxidant enzyme values and the total antioxidant activity. The cardio-protection provided by pomiferin treatment results from the suppression of oxidative stress and correlates with the improved ventricular function.