Acta Vet. Brno 2007, 76: 399-404

In vitro Maturation and Fertilization of Riverine Buffalo Follicular Oocytes in Media Supplemented with Oestrus Buffalo Serum and Hormones

H. Jamil, H. A. Samad, N. U. Rehman, Z. I. Qureshi, L. A. Lodhi

Department of Animal Reproduction, Faculty of Veterinary Science, University of Agriculture, Faisalabad, Pakistan

Received August 11, 2006
Accepted August 27, 2007

Effects of two maturation media (TCM-199 and Ham's F-12) with and without the addition of oestrus buffalo serum (OBS) and hormones (FSH, LH, E2) on the maturation rate of buffalo follicular oocytes were evaluated. The results revealed a significant (P < 0.05) increase in the maturation rate when the OBS and hormones were added to TCM-199 than in Ham's F-12 medium. The percentage of maturation rates in TCM-199 + hormones + OBS, TCM-199 + hormones, TCM-199 + OBS and TCM-199 were 77.44, 55.17, 62.28 and 26.62 percent, respectively. While in Ham's F-12 + OBS + hormone, Ham'F-12 + hormone, Ham's F-12 + OBS and Ham's F-12 were 32.85, 27.52, 31.38 and 13.46 percent, respectively. A significantly higher (P < 0.05) fertilization rate was recorded for modified Ca2+ free Tyrode's medium (63.72%) than in TALP (10.9%) and IVF-TL (32.18%). Thus, TCM-199 containing hormones and OBS appeared better for in vitro maturation, whereas modified Ca2+ free tyrode's medium was found to be more suitable for in vitro fertilization of buffalo follicular oocytes.