Acta Vet. Brno 2007, 76: 579-584
Evaluation of Immunoglobulin Production during Tularaemia Infection in BALB/c Mouse Model
The chromatographic technique was used for assay of time-dependent content of antibodies in mice BALB/c infected by tularaemia. The assay was consequently performed on two types of chromatographic sorbents. The first was commercial CBindTM specific for serum immunoglobulins IgM, IgG and IgA. The second was originally prepared sorbent including protein G covalently bound on EnzacrylRAH particles. This sorbent has specificity for immunoglobulin IgG and its subclasses only. Finally, mass concentration of immunoglobulins in serum was determined. Two curves expressing time behavior of immunoglobulin content (immunoglobulin mass concentration vs. days after immunization) were used for finding the proper mathematical function. The function found was sigmoid; subsequently, appropriate constants needed for function solution were evaluated.
Keywords
Francisella tularensis, immunoglobulin, chromatography, protein L, protein G.
