Acta Vet. Brno 2007, 76: 601-604

Two Protocols of Cryopreservation of Goat Semen with the Use of Computer-Assisted Semen Analysis System

R. Kozdrowski, A. Dubiel, W. Bielas, M. Dzięcioł

Department and Clinic of Reproduction, Ruminants Diseases and Animal Health Protection Wrocław University of Environmental and Life Sciences, Wrocław, Poland

Received February 26, 2007
Accepted October 2, 2007

The objective of the study was a comparison of two protocols of goat semen cryopreservation with the use of computer-assisted semen analysis system. Twenty ejaculates obtained with electroejaculation method were assessed. Each ejaculate was divided in half and frozen according to two protocols. In protocol I semen was centrifuged in order to remove its plasma and diluted in Tris buffer extender containing glucose, citric acid and glycerol with 20% addition of egg yolk. Protocol II did not include removal of plasma and the extender contained 1.5% egg yolk. It was shown that the removal of semen plasma improved motility of goat spermatozoa following freezing/thawing with respect to the following motility indicators: motility, average path velocity, amplitude of lateral head displacement at p < 0.05, and straight velocity, straightness and linearity at p < 0.01. In conclusion, the removal of semen plasma through centrifugation improved motility properties of goat semen following the freezing/thawing procedure.