Acta Vet. Brno 2007, 76: 61-66

Effects of Metazachlor on Vitellogenin Induction in Zebrafish (Danio rerio)

J. Jurčíková1, P. Mikula2, R. Dobšíková2, D. Némethová3,4, Z. Svobodová2

1Institute of Public Health in Ostrava, Ostrava, Czech Republic
2Department of Veterinary Public Health and Toxicology, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic
3Institute of Biostatistics and Analyses, Masaryk University, Brno, Czech Republic
4Research Centre for Environmental Chemistry and Ecotoxicology, Masaryk University, Brno, Czech Republic

Received July 9, 2007
Accepted October 11, 2007

The influence of metazachlor on vitellogenesis in juvenile (20 days old) zebrafish (Danio rerio) was investigated after ambient water exposure to concentrations of 0.1, 1.0 and 5.0 mg l-1 of the chloroacetanilide herbicide Butisan 400 SC containing approximately 35.6% (w/w) metazachlor. After 20 days of exposure, vitellogenin concentrations in whole-body homogenates of the fish were measured by direct sandwich ELISA. The results were compared to vitellogenin concentrations in fish from both negative (no exposure) and positive (exposed to natural oestrogen 17β-oestradiol) control groups. Exposure to Butisan 400 SC at a concentration of 5.0 mg l-1 induced vitellogenin synthesis significantly compared to the control fish (p < 0.05). The oestrogenic effect of 17β-oestradiol was confirmed.