Acta Vet. Brno 2008, 77: 39-44

Influence of Linseed and Fish Oil on Metabolic and Immunological Indicators of Laying Hens

M. Švedová1, L. Vaško1, A. Trebunová1, R. Kašteľ1, M. Tučková2, M. Čertík3

1Department of Chemistry, Biology, and Biochemistry,
2Ist Internal Clinic, University of Veterinary Medicine in Košice, Slovak Republic
3Slovak University of Technology, Faculty of Chemical and Food Technology, Department of Biochemical Technology, Bratislava, Slovak Republic

Received March 5, 2007
Accepted November 15, 2007

The metabolic and immunological indicators were determined in the blood of laying hens of ISA BROWN hybrid divided into three groups, the control group and two experimental groups. The administration of n-3 polyunsaturated fatty acids (PUFA) in the form of linseed (Group 1) and fish oil (Group 2) and α-tocopherol as antioxidant to laying hens resulted in a significant increase in concentrations of high density lipoproteins (HDL) cholesterol (P < 0.05), eicosapentaenoic (EPA), docosahexaenoic (DHA) and α-linolenic acids (AA) in blood in comparison to the control group. Significantly lower levels of cholesterol (CHOL) were determined in both experimental groups at the third sampling (P < 0.05) and arachidonic acid (AA) in the fish group (P < 0.01). The metabolic activity of phagocytes and polyclonal activation of lymphocytes showed no significant differences and remained within the physiological range. Oral administration of n-3 PUFA showed no significant increase of the immune response of experimental animals.