Acta Vet. Brno 2008, 77: 401-406

Indirect Enzyme Linked Immunosorbent Assay for Diagnosis of Brucellosis in Buffaloes

R. Munir1, S. T. Ur Rehman2, R. Kausar1, S. M. Saqlan Naqvi2, U. Farooq1

1Animal Health Laboratories, Animal Sciences Institute, National Agricultural Research Centre, Park Road, Islamabad, Pakistan
2Department of Biochemistry, University of Arid Agriculture, Muree Road, Rawalpindi, Pakistan

Received November 20, 2007
Accepted April 14, 2008

Brucellosis is an important zoonotic disease causing significant economic losses worldwide. Early detection of this disease is essential for its control and eradication. Presently, an Indirect Enzyme Linked Immunosorbent Assay (I-ELISA) was developed using lipopolysaccharide (LPS) as antigen and compared with the commercial kit using one hundred negative and positive sera each from buffaloes. The agreement for the positive result between the developed and commercial I-ELISA was 78% and for the negative it was 100%. At 52.49%, 53.09%, 53.26%, 53.86% and 53.94% cut off the sensitivity was 100%, 100%, 97.53%, 88.93% and 86.42%, while the specificity was 84.03%, 84.87%, 85.71%, 87.39% and 87.39%, respectively, for developed I-ELISA. This developed test can be used for the screening of herds as the relative sensitivity is higher.