Immunomodulating Effect of Methisoprinol on the Pronephros Macrophage and Lymphocyte Activity after Suppression Induced by Infectious Haematopoietic Necrosis Virus (IHNV) in Rainbow Trout (<i>Oncorhynchus mykiss</i>)

Siwicki, A. K.; Małaczewska, J.; Kazun, B.; Wójcik, R.

doi:10.2754/avb200877040631

Acta Veterinaria > Archive > 2008, Vol. 77 > Issue 4 > Immunomodulating Effect of Methisoprinol…

Acta Vet. Brno 2008, 77: 631-635

https://doi.org/10.2754/avb200877040631

Immunomodulating Effect of Methisoprinol on the Pronephros Macrophage and Lymphocyte Activity after Suppression Induced by Infectious Haematopoietic Necrosis Virus (IHNV) in Rainbow Trout (Oncorhynchus mykiss)

A. K. Siwicki^1,2, J. Małaczewska², B. Kazun¹, R. Wójcik²

¹Department of Fish Pathology and Immunology, Inland Fisheries Institute in Olsztyn, Poland
²Department of Microbiology and Clinical Immunology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland

Received March 17, 2008
Accepted July 7, 2008

The purpose of this in vitro study was to determine the influence of methisoprinol on the activity of pronephros macrophages and lymphocytes after suppression induced by infectious haematopoietic necrosis virus (IHNV). For this study IHNV-free rainbow trout were used. Pronephros from 20 fish were removed and single leukocyte suspensions were separated. The IHNV significantly (P < 0.05) decreased the respiratory burst activity and potential killing activity of pronephros macrophages and proliferative response of lymphocytes stimulated by mitogens ConA and LPS. The results of our in vitro study showed that methisoprinol at a concentration of 50 mg/ml modulated (restored) the metabolic and potential killing activity of macrophages and proliferative response of lymphocytes suppressed by IHNV.