Serum thromboxane B<sub>2</sub> (TXB<sub>2</sub>) Determination is Influenced by Sample Incubation Temperature in Healthy Beagle Dogs

Jerin, Aleš; Seliškar, Alenka; Lukanc, Barbara; Butinar, Janoš; Svete, Alenka Nemec

doi:10.2754/avb200978020223

Acta Veterinaria > Archive > 2009, Vol. 78 > Issue 2 > Serum thromboxane B₂…

Acta Vet. Brno 2009, 78: 223-228

https://doi.org/10.2754/avb200978020223

Serum thromboxane B₂ (TXB₂) Determination is Influenced by Sample Incubation Temperature in Healthy Beagle Dogs

Aleš Jerin¹, Alenka Seliškar², Barbara Lukanc², Janoš Butinar³, Alenka Nemec Svete²

¹The University Medical Centre Ljubljana, Institute of Clinical Chemistry and Biochemistry, Ljubljana, Slovenia
²Clinic for Surgery and Small Animal Medicine, Veterinary Faculty, University of Ljubljana, Slovenia
³Clinic for Surgery and Small Animal Medicine, Veterinary Faculty, University of Ljubljana, Slovenia (at the time the work was done); present address: Animal Hospital Postojna, Slovenia

Received June 13, 2008
Accepted November 12, 2008

The measurement of serum thromboxane B₂ (TXB₂) production by platelets is a specific test for assessment of platelet cyclooxygenase (COX-1) activity following administration of non-steroidal anti-inflammatory drugs (NSAIDs). The aim of this study was to investigate the influence of sample incubation at 37 °C for one hour on serum TXB₂ concentration in comparison with incubation at room temperature. A total of 54 blood samples for serum TXB₂ measurements were collected from six healthy beagle dogs into two separate serum tubes. While one group of tubes was incubated in a 37 °C water bath, the second group of tubes was left to coagulate at room temperature, both for one hour. Serum TXB₂ concentrations were measured by ELISA. The mean concentration (± SD) of serum TXB₂ in the group of samples that were incubated at 37 °C was significantly (P < 0.0001) higher compared to the group of samples incubated at room temperature, 1098 ± 346 μg/l and 550 ± 257 μg/l, respectively. The results of the study provide the information on serum TXB₂ concentration in healthy beagle dogs and demonstrate that validated methods for assessment of COX-1 activity by measurement of serum TXB₂ should be used in order to make results more reliable and comparable between different studies. The results of this study might be of great help in planning NSAID studies in dogs by providing the information that TXB₂ generation by platelets is influenced profoundly by incubation temperature.