Acta Vet. Brno 2009, 78: 535-548

The Effects of Sediments Burdened by Sewerage Water Originating in Car Batteries Production in the Klenice River (CZ)

Petra Beránková1, Karl-Werner Schramm2, Martin Bláha1, Jan Rosmus3, Pavel Čupr4

1University of South Bohemia in České Budějovice, Research Institute of Fish Culture and Hydrobiology in Vodňany, Czech Republic
2German Research Centre for Environmental Health, Institute of Ecological Chemistry, München, Germany
3Prague State Veterinary Institute, Prague, Czech Republic
4Research Centre for Environmental Chemistry and Ecotoxicology, Faculty of Science, Masaryk University, Brno, Czech Republic

Received May 26, 2008
Accepted April 6, 2009

The aim of this work was to perform tests of genotoxicity and toxicity on samples of riverine sediments from a location subject to motor industry load (car battery production). Together with sediment samples we also collected benthos, biofilm and juvenile fish. Concentration of lead was established in all the samples since the sewage waters discharged from the car battery production plant are heavily polluted with lead. Genotoxicity was tested with two tests of genotoxicity: the SOS chromotest and the Escherichia coli WP2 test. The toxicity of sediments was tested with a test of toxicity performed on a water crustacean Daphnia magna. A profound toxic influence upon benthic organisms was found; a consequence of the river pollution with waste water and flush water from the car battery production plant. This toxic effect was also proven by an aqueous leach from the test performed with Daphnia magna. Both tests of genotoxicity proved a significant genotoxic potential of the sediment samples linked with the growth of the concentration of lead in the sediments (up to 647 mg kg-1). The content of lead also increased in the biofilm (up to 3.37 mg kg-1 of dry mass) as well as in the fish bodies (up to 804.5 mg kg-1 of dry mass). This thesis is the first study of the load imposed on this river as a consequence of the waste water and flush water discharge from the motor industry production plant (car battery production).