Modulation of Biochemical and Haematological Indices of Silver Carp (<i>Hypophthalmichthys molitrix</i> Val.) Exposed to Toxic Cyanobacterial Water Bloom

Kopp, Radovan; Palíková, Miroslava; Navrátil, Stanislav; Kubíček, Zdenek; Ziková, Andrea; Mareš, Jan

doi:10.2754/avb201079010135

Acta Veterinaria > Archive > 2010, Vol. 79 > Issue 1 > Modulation of Biochemical and…

Acta Vet. Brno 2010, 79: 135-146

https://doi.org/10.2754/avb201079010135

Modulation of Biochemical and Haematological Indices of Silver Carp (Hypophthalmichthys molitrix Val.) Exposed to Toxic Cyanobacterial Water Bloom

Radovan Kopp^1,2, Miroslava Palíková², Stanislav Navrátil³, Zdenek Kubíček⁴, Andrea Ziková^1,2, Jan Mareš¹

¹Department of Fisheries and Hydrobiology, Mendel University in Brno, Czech Republic
²Institute of Botany, Czech Academy of Sciences, Brno, Czech Republic
³Department of Veterinary Ecology and Environmental Protection, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic
⁴Department of Clinical Biochemistry, Hospital Třinec, Třinec, Czech Republic

Received December 17, 2008
Accepted June 30, 2009

The aim of this study was to evaluate the influence of toxic cyanobacterial water bloom on the blood indices of two-year-old silver carp (Hypophthalmichthys molitrix Val.). Experimental fish (336 ± 32 mm length, 365 ± 112 g weight) were exposed to a natural population of cyanobacterial water bloom (mainly Microcystis aeruginosa and M. ichthyoblabe) that contained microcystins (total concentration 133–284 μg g^-1 (DW), concentration in water 2.8–7.4 μg l^-1). Haematological indices showed marked changes in fish exposed to the cyanobacterial population compared to the control group. Statistical evaluation of the influence of cyanobacterial water bloom on biochemical and haematological indices of silver carp showed a distinct decrease of albumin, alkaline phosphatase, cholesterol, glucose, total protein, creatinine, lactate, lactate dehydrogenase, phosphorus, iron, cholinesterase, haemoglobin, haematocrit, erythrocyte and leukocyte compared to the control. Values of alanine aminotransferase were significantly increased compared to the control. After exposure to the cyanobacterial water bloom, the silver carp were kept in pure water for monitoring the persistence of biochemical and haematological indices. Influence of the cyanobacterial population on values of albumin, alkaline phosphatase, cholesterol, total protein, glucose, creatinine, phosphorus, iron, cholinesterase, erythrocyte and haematocrit persisted up to 28 days after the end of exposure. Duration of exposure, toxicity and density of cyanobacterial water bloom had an important impact on individual biochemical and haematological indices.