Acta Vet. Brno 2010, 79: 277-280

Detection of Brucella abortus in Bovine Milk by Polymerase Chain Reaction

Ayman Al-Mariri, Nermeen Haj-Mahmoud

Department of Molecular Biology and Biotechnology, Atomic Energy Commission, Damascus, Syria

Received October 25, 2008
Accepted December 3, 2009

This study compares milk ring test and three different polymerase chain reaction techniques (direct DNA extraction by column purification system, alkaline DNA extraction, and filtrated milk), in order to identify Brucella abortus infection in bovine milk. Milk ring test sensitivity and specificity were 72% and 80%, respectively. While specificity of the three polymerase chain reaction techniques was 100%; sensitivity was 92%, 88% and 100%, respectively, for the three polymerase chain reaction procedures. We conclude that the filtered animal’s milk polymerase chain reaction is the best procedure to make the diagnosis of B. abortus infections.


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