Acta Vet. Brno 2010, 79: 277-280

https://doi.org/10.2754/avb201079020277

Detection of Brucella abortus in Bovine Milk by Polymerase Chain Reaction

Ayman Al-Mariri, Nermeen Haj-Mahmoud

Department of Molecular Biology and Biotechnology, Atomic Energy Commission, Damascus, Syria

Received October 25, 2008
Accepted December 3, 2009

References

1. Alton GG, Jones LM, Angus RD, Verger JM 1988: Techniques for the Brucellosis Laboratory. INRA. Paris.
2. Hamdy ME, Amin AS 2002: Detection of Brucella species in the milk of infected cattle, sheep, goats and camels by PCR.Vet J 163: 299-305 <https://doi.org/10.1053/tvjl.2001.0681>
3. Boschiroli ML, Fouonbne V, O’Callaghan D 2001: Brucellosis: a worldwide zoonosis. Curr Opin Microbiol 4: 58-64 <https://doi.org/10.1016/S1369-5274(00)00165-X>
4. Bricker BJ 2002: PCR as a diagnostic tool for brucellosis. Vet Microbiol 90: 435-446 <https://doi.org/10.1016/S0378-1135(02)00228-6>
5. Cortez A, Scarcelli E, Soares RM, Heinemann MB, Sakamoto SM, Genovez ME, Ferreira F, Richtzenhain LJ 2001: Detection of Brucella DNA from aborted bovine foetuses by polymerase chain reaction. Aust Vet J 79: 500-501 <https://doi.org/10.1111/j.1751-0813.2001.tb13025.x>
6. Daly P, Collier T, Doyls S 2002: PCR-ELISA detection of Escherichia coli in milk. Lett Appl Microb 34: 222-226 <https://doi.org/10.1046/j.1472-765x.2002.01074.x>
7. Darwesh M, Benkirane A 2001: Field investigations of brucellosis in cattle and small ruminants in Syria, 1990-1996. Rev Sci Tech Off Int Epiz 20: 769-775 <https://doi.org/10.20506/rst.20.3.1313>
8. European Commission 2002: Regulation No. 535/2002 of 21 March 2002 amending Annex C to Council Directive 64/432/EEC and amending decision 2000/330/EC. Off J Eur Commun L 80: 22-28
9. FAO 1998: A Perspective of Brucellosis surveillance in north Africa and the Middle East: Studies in Algeria - Iran - Kuwait - Morocco - Oman - Sudan and Syria. January 1998; GCP/REM/059/IFA. FAO Consultancy mission report.
10. Farrell ID 1974: The development of a new selective medium for the isolation of Brucella abortus from contaminated sources. Res Vet Sci 16: 280-286 <https://doi.org/10.1016/S0034-5288(18)33726-3>
11. Fiori PL, Mastrandrea S, Rappelli P, Cappuccinelli P 2000: Brucella abortus infection acquired in microbiology laboratories. J Clin Microbiol 38: 2005-2006
12. Garin-Bastuji B, Blasco JM 2004: Caprine and ovine brucellosis (excluding B. ovis). In: Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, fifth ed. OIE pp. 598-606
13. Godfroid J, Saegerman C, Wellemans V, Walravens K, Letesson J-J, Tibor A, McMillan A, Spencer S, Sanna M, Bakker D, Pouillot R, Garin-Bastuji B 2002: How to substantiate eradication of bovine brucellosis when aspecific serological reactions occur in the course of brucellosis testing. Vet Microbiol 90: 461-477 <https://doi.org/10.1016/S0378-1135(02)00230-4>
14. Gupta VK, Deepak K, Vermaa PK, Routa SV, Singha and Vihana VS 2006: Polymerase chain reaction (PCR) for detection of Brucella melitensis in goat milk. Small Rum Res 65: 79-84 <https://doi.org/10.1016/j.smallrumres.2005.05.024>
15. Leal-Klevezas DS, Martinez-Vazquez IO, Garcia-Cantu J, Lopez-Merino A, Martinez-Soriano JP 2000: Use of polymerase chain reaction to detect Brucella abortus biovar 1 in infected goats. Vet Microbiol 75: 91-97 <https://doi.org/10.1016/S0378-1135(00)00200-5>
16. Leyla G, Kadri G, U¨ Mran O 2003: Comparison of polymerase chain reaction and bacteriological culture for the diagnosis of sheep brucellosis using aborted fetus samples. Vet Microbiol 93: 53-61 <https://doi.org/10.1016/S0378-1135(02)00442-X>
17. Moreno E, Cloeckret A, Moriyon I 2002: Brucella evolution and taxonomy. Vet Microbiol 90: 209-227 <https://doi.org/10.1016/S0378-1135(02)00210-9>
18. Noriello S 2004: Laboratory-acquired brucellosis. Emerg Infect Dis 10: 1848-1850
19. O’Leary S, Sheahan M, Sweeney T 2006: Brucella abortus detection by PCR assay in blood, milk and lymph tissue of serologically positive cows. Res Vet Sci 81: 170-176 <https://doi.org/10.1016/j.rvsc.2005.12.001>
20. OIE Manual 2004: Bovine brucellosis. In: Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, OIE, World Organisation for Animal Health, Part 2 (Chapter 2.3.1).
21. Romero C, Lopez-Goni I 1999: Improved method for purification of bacterial DNA from bovine milk for detection of Brucella spp. by PCR. Appl Environ Microbiol 65: 3735-3737
22. Zvizdic S, Cengic D, Bratic M, Mehantic S, Pinjo F, Hamzic S 2006: Brucella melitensis: review of the human infection case. Bosn J Basic Med Sci 6: 15-18. <https://doi.org/10.17305/bjbms.2006.3203>
23. WHO 2004: Brucellosis. World Health Organization Essential Medicines Library EMLib (accessed 03/12/2004)
front cover
  • ISSN 0001-7213 (printed)
  • ISSN 1801-7576 (electronic)

Current issue

Archive