Acta Vet. Brno 2010, 79: 377-383

Impact of Vitamins C and E Dietary Supplementation on Leukocyte Profile of Layer Hens Exposed to High Ambient Temperature and Humidity

Joachim Joseph Ajakaiye, Alcides Perez-Bello, Angel Mollineda-Trujillo

Department of Zootechnics and Veterinary Medicine, Universidad Central “Marta Abreu” de Las Villas, Santa Clara, Villa Clara, Cuba


1. Altan O, Altan A, Cabuk M, Bayraktar H 2000: Effects of heat stress on some blood parameters in broilers. Turk J Vet Anim Sci 24: 145-148
2. AOAC 1990: Association of Official Analytical Chemists. Official methods of analysis. 15th Ed. Washington D.C. pp. 1298-1930
3. Balnave D 2004: Challenges of accurately defining the nutrient requirements of heat-stressed poultry. In: World’s Poultry Science Association Invited Lecture. Poult Sci 83: 5-14 <>
4. Ben-Nathan D, Heller ED, Perek M 1976: The effect of short heat stress upon leukocyte count, plasma corticosterone level, plasma and leukocyte ascorbic acid content. Br Poult Sci 17: 481-485 <>
5. Beuving G, Jones RB, Blokhuis HJ 1989: Adrenocortical and heterophil/lymphocyte responses to challenge in hens showing short or long tonic immobility reactions. Br Poult Sci 30: 175-184 <>
6. Bouraoui R, Lahmar M, Majdoub A, Djemali M, Belyea R, 2002: The relationship of temperature-humidity index with milk production of dairy cows in a Mediterranean climate. Anim Res 51: 479-491 <>
7. Campbell TW 1995: Avian hematology. In: Campbell TW (ED.): Avian hematology and cytology. Iowa State University Press, Ames, Iowa, pp. 3-19
8. Daghir NJ 2009: Nutritional strategies to reduce heat stress in broilers and broiler breeders. Lohmann Information 44: 6-15
9. David ED, Lindquist WD 1982: Determination of the specific gravity of certain helminthes eggs using sucrose density gradient centrifugation. J Parasitol 68: 916-919 <>
10. Donkoh A 1989: Ambient temperature: A factor affecting performance and physiological response of broiler chickens. Intl J Biomet 33: 259-265 <>
11. Duncan DB 1955: Multiple range and multiple F tests. Biometrics 11: l-42 <>
12. German JB, Traber MG 2001: Nutrients and oxidation: Actions, transport, and metabolism of dietary antioxidants. In: Handbook of vitamins. 3rd Ed Rucker RB, Suttie JW, McCormick DB, Machlin LJ Marcel Dekker Inc New York, pp. 569-588
13. Gey KF 1998: Vitamins C plus E and interacting co-nutrients required for optimal health. BioFactors 7: 113-174
14. Gray HG, Paradis TJ, Chang PW 1989: Physiological effects of adrenocorticotropic hormone and hydrocortisone in laying hens. Poult Sci 68: 1710-1713 <>
15. Gross WB, Siegel PB, Dubose RT 1980: Some effects of feeding corticosterone to chickens. Poult Sci 59: 516-522 <>
16. Gross WB, Siegel HS 1983: Evaluation of the heterophil/lymphocyte ratio as a measure of stress in chickens. Avian Dis 27: 972-979 <>
17. Heller ED, Nathan DB, Perek M 1979: Short heat stress as an immunostimulant in chicks. Avian Pathol 8: 195-203 <>
18. Holik V 2009: Management of laying hens to minimize heat stress. Lohmann Information 44: 16-29
19. Karaman S, Tarhan S, Ergunes G 2007: Analysis of indoor climatic data to assess the heat stress of laying hens. IJNES 1: 65-68
20. Kevin C 1982: Vitamin E in poultry production. 1st Iberian Symposium on aviculture. In: ROCHE Information Service, Animal Nutrition Department, Lisbon, Portugal. pp. 1-6.
21. Maurice DV, Lightsey SF, Abudabos A, Toler JE 2002: Factors affecting ascorbic acid biosynthesis in chickens: III. Effect of dietary fluoride on L-gulonolactone oxidase activity and tissue ascorbic acid (AsA) concentration. J Anim Physiol Anim Nutr 86: 383-388 <>
22. Maxwell MH, Robertson GM 1998: The avian heterophil leukocyte: a review. World’s Poult Sci J 54: 155-178 <>
23. Maxwell MH, Robertson GM, Mitchell MA, Carlisle AJ 1992: The fine structure of broiler chicken blood cells, with particular reference to basophils, after severe heat stress. Comparative Hematol Intl 2: 190-200 <>
24. Maxwell MH 1993: Avian blood leukocyte responses to stress. World’s Poult Sci J 49: 34-43 <>
25. McFarlane JM, Curtis SE 1989: Multiple concurrent stressors in chicks. 3. Effects on plasma corticosterone and the heterophil: lymphocyte ratio. Poult Sci 68: 522-527 <>
26. Minka NS, Ayo JO 2008: Haematology and behavior of pullets transported by road and administered ascorbic acid during the hot-dry season. Res Vet Sci 85: 389-393 <>
27. Mitchell MA, Kettlewell PJ, Maxwell MH 1992: Indicators of physiological stress in broiler chickens during road transportation. Anim Welfare 1: 91-103
28. Mogenet LY, Youbicier-Simon BJ 1998: Determination of reliable biochemical parameters of heat stress, and application to the evaluation of medications: example of erythromycin. In: Proceedings of 10th European Poultry Conference, Jerusalem, Israel, pp. 538-541
29. Mujahid A, Yoshiki Y, Akiba Y, Toyomizu M 2005: Superoxide radical production in chicken skeletal muscle induced by acute heat stress. Poult Sci 84: 307-314 <>
30. NRC 1994: Nutrient requirements of poultry. 9th rev. Ed. National Academy of Science Washington D.C, pp. 19-26
31. Nworgu FC, Ogungbenro SA, Solesi KS 2007: Performance and some blood chemistry indices of broiler chicken served fluted pumpkin (Telfaria occidentalis) leaves extract supplement. American-Eurasian J Agric Environ Sci 2: 90-98
32. Ramnath V, Rekha PS, Sujatha KS 2008: Amelioration of heat stress induced disturbances of antioxidant defense system in chicken by Brahma Rasayana. Evidence- Based Compl Alt Med 5: 77-84 <>
33. SAS USER’S GUIDE 1985: Statistics In: Joyner SP. Ed. The GLM procedure. SAS Inst Inc Cary Newcastle
34. Shane SM 2005: Health and performance of poultry in tropical climates. In: ASA handbook on poultry diseases. 2nd Ed published by American Soybean Association, Singapore, pp. 13-20
35. Siegel HS 1980: Physiological stress in birds. Bio Science 30: 529-533
36. Thaxton P, Sadler CR, Glick B 1968: Immune response of chickens following heat exposure or injections with ACTH. Poult Sci 47: 264-266 <>
37. UECAN 2002: Technical instructions for commercial layers and parent breeders. La Habana, Cuba (In Spanish)
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