Acta Vet. Brno 2010, 79: 443-448
Modulation of Tularemia Disease Progress by the Bisquaternary Pyridinium Oxime HI-6
Cholinesterase reactivator HI-6 is a drug commonly used to treat individuals exposed to nerve agents. Recent experiments proved HI-6 impact on parasympathetic response and impact on the nervus vagus associated cholinergic anti-inflammatory pathway is hypothesized here. The modulation effect of HI-6 was studied on BALB/c mice infected with Francisella tularensis, the bacteria causing tularemia. Cultivation test in vitro confirmed weak bacteriostatic effects of HI-6. Results in experiments revealed intriguing effect differences resulting from HI-6 administration to mice. While the HI-6 dose of 7 mg per animal (pro toto) had no significant effects on infection progress, lower dose of 8 μg of HI-6 pro toto reduced mice mortality caused by tularemia infection compared to control group of mice infected only with F. tularensis (survival curves were compared by the logrank test, chi square = 4.335, df = 1, P = 0.0373). The effect observed in mice exceeded the effect provided in vitro on bacterial cultures. Though the exact molecular mechanism of HI-6 modulation during infection should be investigated, HI-6 is seems to be suitable as immunomodulans.
Keywords
Asoxime, immunity, infection progress, Francisella tularensis, cholinesterase, Inflammation.
