Acta Vet. Brno 2010, 79: 453-457

Gene Expression of the Endothelin-1 in Vasospastic Flap Pedicle – an Experimental Study on a Porcine Model

Petr Hýža1, Libor Streit1, Eduard Gopfert2, Daniel Schwarz3, Michal Masařík4, Michal Jurajda4, Anna Vašků4, Jiří Veselý1

1Clinic of Plastic and Aesthetic Surgery, Masaryk University, Brno, Czech Republic
2Veterinary Research Institute, Brno, Czech Republic
3Institute of Biostatistics and Analyses, Masaryk University, Brno, Czech Republic
4Department of Pathological Physiology, Masaryk University, Brno, Czech Republic

The aim of this study was to evaluate the amount of Endothelin-1 (ET-1) gene expression in the vasospastic vessel of the flap pedicle to prove or disprove the role of ET-1 gene expression in pathogenesis of mechanically induced vasospasm. The vasospasm was induced by the tension on the pedicle of the pedicled caudal superficial epigastric flap on 8 pigs. Laser Doppler was used for peripheral blood flow measurement. Specimens from the vasospastic vessel (group of specimens B) and from the flap border with no vasospasm (control group A) were taken 2 h after the stimulus initiation. Detection of ET-1 mRNA by Quantitative Real-Time RT-PCR was performed. β-actin was selected as an acceptable reference gene. Relative gene expression data were given as the n-fold change in transcription of target genes normalized to the endogenous control. Relative gene expressions and time indicators of vasospasm were compared in both groups. No significant difference of the ET-1 gene expressions was found between groups A and B (p = 0.505). No correlation between the duration of vasospasm and ET-1 gene expression was found as well (p = 0.299). In conclusion, the expression of the ET-1 gene in the mechanically induced vasospastic vessel of the pedicled flap was not significantly increased. In this study, the causality of the vasospasm pathogenesis and gene expression of ET-1 was not proven.


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