Acta Vet. Brno 2010, 79: 587-592

Acute Toxicity of the Preparation PAX-18 for Juvenile and Embryonic Stages of Zebrafish (Danio rerio)

Stanislava Mácová, Lucie Plhalová, Zuzana Široká, Petra Doleželová, Vladimíra Pištěková, Zdeňka Svobodová

Department of Veterinary Public Health and Toxicology, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic

The preparation PAX-18 is a coagulation agent, which is used in water and wastewater treatment facilities and for the treatment of natural waters. The active compound is polyaluminium chloride (9% of Al). The application to the water environment could present a potential risk to different developmental stages of fish. The aim of this study was to assess and compare the toxicity of the preparation PAX-18 for embryonic and juvenile developmental stages of zebrafish (Danio rerio). The acute toxicity tests with juvenile fish aged 2–3 months were conducted according to the method OECD No. 203 in 5 series. For embryo toxicity test the method OECD No. 212 was used in 5 series. The semistatic methods were selected. The results of toxicity tests (the number of dead individuals at particular test concentrations) were subjected to probit analysis using the EKO-TOX 5.2 programme to determine LC50 values of PAX. The LC50 mean value of PAX for juvenile D. rerio was 749.7 ± 30.6 mg·l-1 (67.5 ± 2.8 mg·l-1 of Al) and 731.5 ± 94.1 mg·l-1 (65.8 ± 8.5 mg·l-1 of Al) for embryonic stages of D. rerio. The sensitivity of juvenile and embryonic stages to PAX were comparable (p > 0.01). The acute toxicity values of PAX-18 found in tests on D. rerio were 6–13 × higher than the concentration which is usually applied to waters (5–10 mg·l-1 of Al). Therefore, the acute toxicity effect on fish can be considered minimal.


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