Acta Vet. Brno 2011, 80: 113-118

https://doi.org/10.2754/avb201180010113

Phosphorylation of histone H2AX as an indicator of received dose of gamma radiation after whole-body irradiation of rats

Radim Havelek1, Martina Řezáčová1, Zuzana Šinkorová2, Lenka Zárybnická2, Aleš Tichý1,2, Jiřina Vávrová2

1Department of Medical Biochemistry, Faculty of Medicine in Hradec Králové, Charles University in Prague, Czech Republic
2Department of Radiobiology, University of Defence, Faculty of Military Health Sciences, Hradec Králové, Czech Republic

References

1. Andrievski A, Wilkins RC 2009: The response of gamma-H2AX in human lymphocytes and lymphocytes subsets measured in whole blood cultures. Int J Radiat Biol 85: 369-376 <https://doi.org/10.1080/09553000902781147>
2. Banin S, Moyal L, Shieh S, Taya Y, Anderson CW, Chessa L, Smorodinsky NI, Prives C, Reiss Y, Shiloh Y, Ziv Y 1998: Enhanced phosphorylation of p53 by ATM in response to DNA damage. Science 281: 1674-1677 <https://doi.org/10.1126/science.281.5383.1674>
3. Bassing CH, Alt FW 2004: H2AX may function as an anchor to hold broken chromosomal DNA ends in close proximity. Cell Cycle 3:149-153 <https://doi.org/10.4161/cc.3.2.684>
4. Bekker-Jensen S, Lukas C, Kitagawa R, Melander F, Kastan MB, Bartek J, Lukas J 2006: Spatial organization of the mammalian genome surveillance machinery in response to DNA strand breaks. Cell Biol 173: 195-206 <https://doi.org/10.1083/jcb.200510130>
5. Blakely WF, Brooks AL, Lofts RS, van der Schans GP, Voisin P 2002: Overview of low-level radiation exposure assessment: biodosimetry. Mil Med Feb 167 (2 Suppl): 20-24
6. Finney DG 1947: Probit Analysis. Cambridge University Press (Cambridge). ISBN 978-0-521-13590-0 (digitally printed version 2009) 253p
7. Halicka HD, Huang X, Traganos F, King MA, Dai W, Darzynkiewicz Z 2005: Histone H2AX phosphorylation after cell irradiation with UV-B: relationship to cell cycle phase and induction of apoptosis. Cell Cycle 4: 339-345 <https://doi.org/10.4161/cc.4.2.1486>
8. Huang X, Darzynkiewicz Z 2006: Cytometric assessment of histone H2AX phosphorylation: a reporter of DNA damage. Methods Mol Biol 314: 73-80 <https://doi.org/10.1385/1-59259-973-7:073>
9. Huang X, Okafuji M, Traganos F, Luther E, Holden E, Darzynkiewicz Z 2004: Assessment of histone H2AX phosphorylation induced by DNA topoisomerase I and II inhibitors topotecan and mitoxantrone and by the DNA cross-linking agent cisplatin. Cytometry Part A 58A: 99-110 <https://doi.org/10.1002/cyto.a.20018>
10. Löbrich M, Rief N, Kühne M, Heckmann M, Fleckenstein J, Rübe C, Uder M 2005: In vivo formation and repair of DNA double-strand breaks after computed tomography examinations. Proc Natl Acad Sci USA. 102: 8984-8989
11. Nakamura AJ, Redon CE, Bonner WM, Sedelnikova OA 2009: Telomere-dependent and telomere- independent origins of endogenous DNA damage in tumor cells. Aging 1: 212-218 <https://doi.org/10.18632/aging.100019>
12. Olive PL 2009: Endogenous DNA breaks: γH2AX and the role of telomeres. Aging 1: 154-156 <https://doi.org/10.18632/aging.100025>
13. Redon CE, Dickey JS, Bonner WM, Sedelnikova OA 2009: Gamma-H2AX as a biomarker of DNA damage induced by ionizing radiation in human peripheral blood lymphocytes and artificial skin. Adv Space Res 43: 1171-1178 <https://doi.org/10.1016/j.asr.2008.10.011>
14. Rogakou PE, Pilch DR, Orr AH, Ivanova VS, Bonner WM 1998: DNA Double-stranded breaks induce histone H2AX phosphorylation on serine 139. J Biol Chem 273: 5858-5868 <https://doi.org/10.1074/jbc.273.10.5858>
15. Rogakou PE, Boon C, Redon CE, Bonner WM 1999: Megabase chromatin domain involved in DNA double-strand breaks in vivo. J Cell Biol 146: 905-916 <https://doi.org/10.1083/jcb.146.5.905>
16. Řezáčová M, Tichý A, Vávrová J, Vokurková D, Lukášová E 2008: Is defect in phosphorylation of Nbs1 responsible for high radiosensitivity of T-lymphocyte leukemia cells MOLT-4? Leuk Res 32: 1259-1267 <https://doi.org/10.1016/j.leukres.2007.12.014>
17. Roth Z, Josívko M, Malý V, Trčka V 1962: Statistické metody v experimentální medicíně / Statistic methods in experimental medicine. Státní Zdravotnické Nakladatelství Praha: 589 p.
18. Sak A, Grehl S, Erichsen P, Engelhard M, Grannass A, Levegrün S, Pöttgen C, Groneberg M, Stuschke M 2007: Gamma-H2AX foci formation in peripheral blood lymphocytes of tumor patients after local radiotherapy to different sites of the body: dependence on the dose-distribution, irradiated site and time from start of treatment. Int J Radiat Biol 83: 639-652 <https://doi.org/10.1080/09553000701596118>
19. Vávrová J, Filip S 2002: Radiosenzitivita hematopoetického systému / Radiosensitivity of hematopoietic system. Galén (Praha) ISBN80-7262-200-5, 99s
20. Vilasová Z, Rezácová M, Vávrová J, Tichý A, Vokurková D, Zoelzer F, Řeháková Z, Osterreicher J, Lukášová E 2008: Changes in phosphorylation of histone H2A.X and p53 in response of peripheral blood lymphocytes to gamma irradiation. Acta Biochim Pol 55: 381-390
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