Acta Vet. Brno 2011, 80: 147-156

Intestinal mucin dynamic and leukocytic responses of chickens infected with Eimeria acervulina and fed oregano supplemented diet

Peter Major1, Viera Revajová2, Martin Levkut2, Zuzana Ševčíková2, Viera Spišáková1, Zita Faixová2, Mária Levkutová1, Ivona Kožárová3, Mária Goldová1, Mikuláš Levkut2

1Department of Epizootology and Parasitology, University of Veterinary Medicine and Pharmacy, Košice, Slovak Republic
2Department of Pathological Anatomy and Pathological Physiology, University of Veterinary Medicine and Pharmacy, Košice, Slovak Republic
3Department of Meat Hygiene and Technology, University of Veterinary Medicine and Pharmacy, Košice, Slovak Republic

We studied the effect of oregano supplemented diet on mucin dynamics in small intestine, peripheral blood leukocytes, and jejunal immunocompetent cells in ROSS 308 hybrid broilers infected with Eimeria acervulina chickens’ oocyst. From the day of hatching chicks of groups 1 and 2 were fed a commercial diet without anticoccidial drug, the diet of group 3 was supplemented with oregano (0.707g·kg-1), and that of group 4 with anticoccidial drug (Robenidin hydrochloride – 33 mg·kg-1). Chickens of groups 2, 3, and 4 were inoculated with E. acervulina oocysts (25.103) on day 12. The samples were collected on 3, 10, 17 days post infection (dpi). In blood on 3 dpi significant increase of leukocytes was found in group 3 compared to groups 1, 2, and 4, higher density of IgM+ cells in group 3 than group 2, and on 10 dpi phagocytic activity of group 3 was higher than group 1. Number of jejunal CD4+ and CD8+ cells in group 3 was consistent with values in group 4, despite higher density of E. acervulina meronts on 10 dpi. The quantity of jejunal mucin adherent layer of group 3 was similar to that in group 4. Counts of oocysts in faeces were lower in group 3 than group 2. Results suggest that dietary supplementation of oregano to chickens infected with E. acervulina has a modulating effect on some blood indicators and functions of phagocytes. The beneficial effect of oregano components on jejunal mucin quantity and its turnover is the first finding published in relation to oregano and coccidia.


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