Acta Vet. Brno 2011, 80: 215-218

The effect of thermal shock on morphological characteristics of blood cells in Siberian sturgeon (Acipenser baerii) triploids

Teresa Wlasow, Dorota Fopp-Bayat

Department of Ichthyology, Faculty of Environmental Science and Fisheries, University of Warmia and Mazury in Olsztyn, Poland

The aim of the study was to evaluate the effect of thermal shock on morphotic blood elements in Siberian sturgeon Acipenser baerii triploids. The thermal shock (37 °C for 2 min) was applied in the 18th min after fertilization. Blood was sampled from parallel cultured ten triploids and ten diploids on day 70 after hatching. Ploidy was assessed with the cytogenetic method and measurements of cellular nuclei. In the blood of triploids, significant dominance of immature red blood cells, erythrocytes with a displaced nucleus, microcytes and erythroplastids were observed. The blood of triploids was also characterized by a reduced number of lymphocytes. The percentage of neutrophil and eosinophil granulocytes was elevated; increased share of neutrophil granulocytes with a 4-, 5- or 6-segmented nucleus and eosinophil granulocytes with a nucleus consisting of three and more segments was observed. Disturbances in the picture of red blood cells can be considered as an expression of intensification of end-stage changes in triploids. The response to these changes in the blood of triploid Siberian sturgeon is an increase in the share of polymorphonuclear PMN, cells counted as microphages. Frequent presence of immature red blood cells in triploid Siberian sturgeon is a process that aims at counterbalancing the loss among these blood cells. It is the first report on morphological changes and proportions among blood cells in triploid Siberian sturgeon.


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