Acta Vet. Brno 2012, 81: 3-8

Detection of the Newcastle disease virus and its effect on development of post-vaccination immunity in a commercial flock of laying hens

Jana Jeřábková1, Růžena Juranová2, Kateřina Rosenbergová3,4, Libuše Kulíková2, Alfred Hera1, Petr Lány3,4, Oldřich Kubíček4, Josef Koláček5

1Institute for State Control of Veterinary Biologicals and Medicines, Brno, Czech Republic
2Avian and Exotic Animal Clinic
3Department of Infectious Diseases and Epidemiology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Science Brno, Czech Republic
4National Institute for Nuclear, Chemical and Biological Protection, Kamenna, Czech Republic
5Company Proagro Nymburk a.s., department Opatovice, Czech Republic


1. Alexander DJ 2000: Newcastle disease and other avian paramyxoviruses. Rev Sci Tech 19: 443-462
2. Annex to the EFSA Journal 2007. Available at: Accessed August 25, 2009
3. Awan MA, Otte MJ, James AD 1994: The epidemiology of Newcastle disease in rural poultry: a review. Avian Pathol 23: 405-423
4. Banerjee M, Reed WM, Fitzgerald SD, Panigraphy B 1994: Neurotropic velogenic Newcastle disease in cormorants in Michigan: pathology and virus characterization. Avian Dis 38: 873-878
5. Capua I, Scacchia M, Toscani T, Caporale V 1993: Unexpected isolation of virulent Newcastle disease virus from commercial embryonated fowls´ eggs. J Vet Med B 40: 609-612
6. Collins MS, Bashiruddin JB, Alexander DJ 1993: Deduced amino acid sequences at the fusion protein cleavage site of Newcastle disease viruses showing variation in antigenicity and pathogenicity. Arch Virol 128: 363-370
7. Gohm DS, Thur B, Hofmann MA 2000: Detection of Newcastle disease virus in organs and faeces of experimentally infected chickens using RT-PCR. Avian Pathol 29: 143-152
8. Kant A, Koch G, Van Roozelaar DJ, Balk F, Huurne AT 1997: Differentiation of virulent and non-virulent strains of Newcastle disease virus within 24 hours by polymerase chain reaction. Avian Pathol 26: 837-849
9. Kattenbelt JA, Stevens MP, Gould AR 2006: Sequence variation in the Newcastle disease virus genome. Virus Res 116: 168-184
10. de Leeuw OS, Koch G, Hartog L, Ravenshorst N, Peeters BP 2005: Virulence of Newcastle disease virus is determined by the cleavage site of the fusion protein and by both the stem region and globular head of the haemagglutinin-neuraminidase protein. J Gen Virol 86: 1759-1769
11. Lee YJ, Sung HW, Choi JG, Kim JH, Song CS 2004: Molecular epidemiology of Newcastle disease viruses isolated in South Korea using sequencing of the fusion protein cleavage site region and phylogenetic relationships. Avian Pathol 33: 482-491
12. Liu XF, Wan HQ, Ni XX, Wu YT, Liu WB 2003: Pathotypical and genotypical characterization of strains of Newcastle disease virus isolated from outbreaks in chicken and goose flocks in some regions of China during 1985-2001. Arch Virol 148: 1387-1403
13. OIE 2008: Manual of diagnostic tests and vaccines for terrestrial animals, Newcastle disease: 576-589
14. Pospisil Z, Zendulkova D, Smid B 1991: Unexpected emergence of Newcastle disease virus in very young chicks. Acta Vet Brno 60: 263-270
15. Seal BS, King DJ, Sellers HS 2000: The avian response to Newcastle disease virus. Dev Comp Immunol 24: 257-268
front cover
  • ISSN 0001-7213 (printed)
  • ISSN 1801-7576 (electronic)

Current issue