Acta Vet. Brno 2012, 81: 107-111

A surface plasmon resonance biosensor for direct detection of the rabies virus

Jing Xu1, Jia-yu Wan1, Song-tao Yang1, Shou-feng Zhang1, Na Xu2, Nan Li1, Ji-ping Li1, Hai-ying Wang3, Xue Bai3, Wen-sen Liu1

1Institute of Military Veterinary Science, Academy of Military Medical Science, Changchun, Jilin, P. R. China, Key Laboratory of Jilin province for Zoonosis Prevention and Control, Changchun, Jilin, P. R. China
2Jilin Medical College, Jilin, P. R. China
3College of Animal Science and Veterinary Medicine, Changchun, Jilin, P. R. China

A surface plasmon resonance biosensor chip was constructed for detection of rabies virus. For the construction of the biosensor chip, N protein specific antibody and N protein specific antibody combined with G protein specific antibody of rabies virus were linked on two different flow cells on one CM5 chip, respectively. The chip was tested for the detection of rabies virus antigens using the crude extract of rabies virus from infected BHK cell strain culture. Tenfold serial dilutions of SRV9 strain virus-infected cell cultures were tested by the biosensor chip to establish the detection limit. The limit detection was approximately 70 pg/ml of nucleoprotein and glycoprotein. The biosensor chip developed in this study was employed for the detection of rabies virus in five suspect infectious specimens of brain tissue from guinea pigs; the results were compared by fluorescent antibody test. Surface plasmon resonance biosensor chip could be a useful automatic tool for prompt detection of rabies virus infection.


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