Acta Vet. Brno 2012, 81: 175-182

Cytotoxic activity of the neurotoxin anatoxin-a on fish leukocytes in vitro and in vivo studies

Anna Rymuszka

Department of Physiology and Ecotoxicology, Institute of Biotechnology, John Paul II Catholic University of Lublin, Poland


1. Bumke-Vogt C, Mailahn W, Chorus I 1999: Anatoxin-a and neurotoxic cyanobacteria in German lakes and reservoirs. Environ Toxicol 14: 117-125 <<117::AID-TOX15>3.0.CO;2-V>
2. Edwards C, Beattie KA, Scrimgeour CM, Codd GA 1992: Identification of anatoxin-a in benthic cyanobacteria (blue-green algae) and in associated dog poisonings at Loch Insh, Scotland. Toxicon 30: 1165-1175 <>
3. Gugger M, Lenoir S, Berger C, Ledreux A, Druart JC, Humbert JF, Guette C, Bernard C 2005: First report in a river in France of the benthic cyanobacterium Phormidium favosum producing anatoxin-a associated with dog neurotoxicosis. Toxicon 45: 919-928 <>
4. Harford A, O’Halloran K, Wright P 2005: The effects of in vitro pesticide exposures on the phagocytic function of four native Australian freshwater fish. Aquat Toxicol 75: 330-342 <>
5. Hedman CJ, Krick WR, Karner Perkins DA, Harrahy EA, Sonzogni WC 2008: New measurements of cyanobacterial toxins in natural waters using high performance liquid chromatography coupled to tandem mass spectrometry. J Environ Qual 37: 1817-1824 <>
6. Krienitz L, Ballot A, Kotut K, Wiegand C, Pütz S, Metcalf JS, Codd GA, Pflugmacher S 2003: Contribution of hot spring cyanobacteria to the mysterious deaths of lesser flamingos at lake Bogoria, Kenya. FEMS Microbiol Ecol 43: 141-148 <>
7. Osswald J, Carvalho AP, Claro J, Vasconcelos V 2009: Effects of cyanobacterial extracts containing anatoxin-a and of pure anatoxin-a on early developmental stages of carp. Ecotoxicol Environ Saf 72: 473-478 <>
8. Osswald J, Rellán S, Carvalho AP, Gago A, Vasconcelos V 2007a: Acute effect of anatoxin-a producing cyanobacteria on juvenile fish Cyprinus carpio. Toxicon 49: 693-698 <>
9. Osswald J, Rellán S, Carvalho AP, Gago A, Vasconcelos V 2007b: Toxicology and detection methods of the alkaloid neurotoxin produced by cyanobacteria, anatoxin-a. Environ Int 33: 1070-1089 <>
10. Park HD, Kim B, Kim E, Okino T 1998: Hepatotoxic microcystins and neurotoxic anatoxin-a in cyanobacterial blooms from Korean lakes. Environ Toxicol Water Qual 13: 225-234 <<225::AID-TOX4>3.0.CO;2-9>
11. Pawlik-Skowrońska B, Skowroński T, Pirszel J, Adamczyk A 2004: Relationship between cyanobacterial bloom composition and anatoxin-a and microcystin occurrence in the eutrophic dam reservoir (SE Poland). Pol J Ecol 52: 479-490
12. Rao PVL, Bhattacharya R, Gupta N, Parida MM, Bhaskar ASB, Dubey R 2002: Involvement of caspase and reactive oxygen specie in cyanobacterial toxin anatoxin-a-induced cytotoxicity and apoptosis in rat thymocytes and Vero cells. Arch Toxicol 76: 227-235
13. Rogers EH, Hunter ES, Moser VC, Phillips PM, Herkovitz J, Muńoz L, Hall LL, Chernoff N 2005: Potential developmental toxicity of anatoxin-a, a cyanobacterial toxin. J App Toxicol 25: 527-534 <>
14. Rymuszka A, Sierosławska A 2010: Study on apoptotic effects of neurotoxin anatoxin-a on fish immune cells. Neuroendocrinol Lett 31: 11-15
15. Rymuszka A, Sierosławska A 2011: Effects of neurotoxin – anatoxin-a on common carp (Cyprinus carpio L.) innate immune cells in vitro. Neuroendocrinol Lett 32: 84-88
16. Sivonen K, Jones G 1999: Cyanobacterial toxins. In: Chorus I (Ed.): Cyanotoxins occurrence, causes and consequences. Springer-Verlag, Germany, pp. 41-111
17. Teneva I, Mladenov R, Popov N, Dzhambazov B 2005: Cytotoxicity and apoptotic effects of microcystin-LR and anatoxin-a in mouse lymphocytes. Folia Biol (Praha) 51: 62-67
18. Tort L 2011: Stress and immune modulation in fish. Dev Comp Immunol 35: 1366-1375 <>
19. Viaggiu E, Melchiorre S, Volpi F, Di Corcia A, Mancini R, Garibaldi L, Crichigno G, Bruno M 2004: Anatoxin a toxin in the cyanobacterium Planktothrix rubescens from a fishing pond in northern Italy. Environ Toxicol 19: 191-197 <>
20. Zirger JM, Beattie CE, Mckay DB, Boyd RT 2003: Cloning and expression of zebrafish neuronal nicotinic acetylcholine receptors. Gene Expr Patterns 3: 747-754 <>
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