Acta Vet. Brno 2012, 81: 327-331

https://doi.org/10.2754/avb201281040327

Detection of Mycoplasma hyopneumoniae by ELISA and nested PCR from blood samples and nasal swabs from pigs in Slovakia

Marián Prokeš1, Dagmar Zendulková2, Kateřina Rosenbergová2, František Treml2, Anna Ondrejková1, Zdenek Beníšek1, Róbert Ondrejka1, Ľuboš Korytár1, Eva Slepecká1, Judit Süli3, Eva Haladová4, Dávid Maženský5

1University of Veterinary Medicine and Pharmacy in Košice, Department of Epizootiology and Preventive Veterinary Medicine, Košice, Slovak Republic
2University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Medicine, Department of Infectious Diseases and Microbiology, Brno, Czech Republic
3University of Veterinary Medicine and Pharmacy in Košice, Department of Pharmaceutical and Medical Chemistry, Košice, Slovak Republic
4University of Veterinary Medicine and Pharmacy in Košice, Department of Animal Hygiene and Environment, Košice, Slovak Republic
5University of Veterinary Medicine and Pharmacy in Košice, Department of Anatomy, Košice, Slovak Republic

References

1. Calsamiglia M, Pijoan C, Trigo A 1999: Application of a nested polymerase chain reaction assay to detect Mycoplasma hyopneumoniae from nasal swabs. J Vet Diagn Invest 11: 246-251 <https://doi.org/10.1177/104063879901100307>
2. Damrongwatanapokin T, Damrongwatanapokin S, Pinyochon W, Maneechaivong K 2004: Mycoplasma hyopneumoniae seroprevalence in Thailand during 1997–1998. Proceedings of the 18th IPVS Congress, Hamburg, Germany, 27 June–1 July, 236
3. Dors A, Czyzewska E, Kwit K, Pejsak Z 2012: Seroprevalence of important swine pathogens in Polish farms. Proceedings of the 22th IPVS Congress, Jeju, Korea, 10–13 June, 362
4. Feld N, Quist P, Ahrens P, Friis N, Meyling A 1992: A monoclonal blocking ELISA detecting serum antibodies to Mycoplasma hyopneumoniae. Vet Microbiol 30: 35-46 <https://doi.org/10.1016/0378-1135(92)90092-8>
5. Grosse-Beilage E, Rohde N, Krieter J 2009: Seroprevalence and risk factors associated with seropositivity in sows from 67 herds in north-west Germany infected with Mycoplasma hyopneumoniae. Prev Vet Med 88: 255-263 <https://doi.org/10.1016/j.prevetmed.2008.10.005>
6. Halbur PC, Paul PS, Meng XJ, Lum MA, Andrews JJ, Rathje JA 1996: Comparative pathogenicity of nine US Porcine Reproductive and Respiratory Syndrome Virus (PRSSV) isolates in a five-week-old cesarean-derived, colostrumdeprived pig model. J Vet Diagn Inv 8: 11-20 <https://doi.org/10.1177/104063879600800103>
7. He Y, Xu MJ, Zhou DH, Zou FC, Lin RQ, Yin CC, He XH, Liang R, Liang M, Zhu XQ 2011: Seroprevalence of Mycoplasma hyopneumoniae in pigs in subtropical southern China. Trop Anim Health Prod 43: 695-698 <https://doi.org/10.1007/s11250-010-9755-3>
8. Jackson PGG, Cockcroft PD 2007: Handbook of pig medicine, Saunders Elsevier, USA, 308 pp.
9. Kurth KT, Hsu T, Snook ER, Thacker EL, Thacker BJ, Minion FC 2002: Use of a Mycoplasma hyopneumoniae nested polymerase chain reaction test to determine the optimal sampling sites in swine. J Vet Diagn Invest 14: 463-469 <https://doi.org/10.1177/104063870201400603>
10. Le Potier M, Abiven P, Kobisch M, Crevat D, Desmettre P 1994: A blocking ELISA using a monoclonal antibody for the serological detection of Mycoplasma hyopneumoniae. Res Vet Sci 56: 338-345
11. Lehner S, Meemken D, Nathues H, Grosse-Beilage E 2008: Influence of maternal antibodies induced by sow vaccination on immune response to Mycoplasma hyopneumoniae infectionon in vaccinated and non-vaccinated pigs. Proceedings of the 20th IPVS Congress, Durban, South Africa, 22–26 June, 105
12. Lysenko NV, Milanko AYa, Dubov NS, Zabora NP 1980: Serological survey for porcine enzootic pneumonia in the Ukraine (in Ukrainian). Veterinariya 51: 18-22
13. Mattsson JG, Bergström K, Wallgren P, Johansson KE 1995: Detection of Mycoplasma hyopneumoniae in nose swabs from pigs by in vitro amplification of the 16S rRNA gene. J Clin Microbiol 33: 893-897
14. Morris C, Gardner I, Hietala S, Carpenter T, Anderson R, Parker K 1995: Seroepidemiologic study of natural transmission of Mycoplasma hyopneumoniae in a swine herd. Prev Vet Med 21: 323-337 <https://doi.org/10.1016/0167-5877(94)00384-U>
15. Pilipčinec E, Švický E, Mikula I, Šnirc J 1998: Porcine respiratory disease complex and the interest of bacterial pathogens in its creation (in Slovak). Slov Vet Čas 23: 192-199
16. Point AM, Byrt D, Heap P 1985: Effect of enzootic pneumonia of pigs on growth performance. Aust Vet J 62: 13-18
17. Silin DS, Lyubomska OV, Chung NW 2001: Mycoplasma hyopneumoniae vaccination influence on porcine reproductive and respiratory syndrome virus and Mycoplasma hyopneumoniae coinfection. Acta Vet Brno 70: 413-420 <https://doi.org/10.2754/avb200170040413>
18. Siugzdaite J, Garlaite K 2002: Effect of vaccination against Mycoplasma hyopneumoniae in a pig herd from birth to slaughter. Acta Vet Brno 71: 549-553 <https://doi.org/10.2754/avb200271040549>
19. Thacker E, Halbur P, Ross R, Thanawongnuwech R, Thacker B 1999: Mycoplasma hyopneumoniae potentiation of porcine reproductive and respiratory syndrome virus-induced pneumonia. J Clin Microbiol 37: 620-627
20. Verdin E, Saillard C, Labbé A, Bové JM, Kobisch M 2000: A nested PCR assay for the detection of Mycoplasma hyopneumoniae in tracheobronchiolar washing from pigs. Vet Microbiol 76: 31-40 <https://doi.org/10.1016/S0378-1135(00)00228-5>
21. Villarreal I, Vranckx K, Duchateau L, Pasmans F, Haesebrouck F, Jensen JC, Nanjiani IA, Maes D 2010: Early Mycoplasma hyopneumoniae infections in European suckling pigs in herds with respiratory problems: detection rate and risk factors. Vet Med 55: 318-324 <https://doi.org/10.17221/3017-VETMED>
22. Yagihashi T, Kazama S, Tajima M 1993: Seroepidemiology of mycoplasmal pneumonia of swine in Japan as surveyed by an enzyme-linked immunosorbent assay. Vet Microbiol 34: 155-166 <https://doi.org/10.1016/0378-1135(93)90169-8>
23. Young TF, Ross RF, Drisko J 1983: Prevalence of antibodies to Mycoplasma hyopneumoniae. Am J Vet Res 44: 1946-1948
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  • ISSN 0001-7213 (printed)
  • ISSN 1801-7576 (electronic)

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