Acta Vet. Brno 2013, 82: 131-134

https://doi.org/10.2754/avb201382020131

Gene expression of T lymphocytes of gravid cows during preimplantation

Yousuke Maeda1, Kana Yamamoto1, Hiromichi Ohtsuka1, Takaaki Ando2, Michiko Tomioka3, Masaaki Oikawa1

1Kitasato University, Faculty of Veterinary Medicine, Laboratory of Large Animal Internal Medicine, Japan
2Kagoshima University, School of Joint Faculty of Veterinary Medicine, Laboratory of Veterinary Theriogenology, Japan
3Kitasato University, Faculty of Veterinary Medicine, Laboratory of Large Animal Surgery, Japan

An interaction between the conceptus and the immune system of animals is important during implantation. The aim of this study was to clarify the gene expression of T cell subsets in gravid cows during the preimplantation period. Peripheral blood from 14 Holstein dairy cows was taken 14 days after artificial insemination. Based on the gravidity, cows were divided into gravid (n = 8) and nongravid (n = 6) groups. Mononuclear cells from peripheral blood were stimulated with phytohaemagglutinin and then CD4+, CD8+, and WC1+ γδ T cell subsets were isolated using magnetic cell sorting. The expression of interferon γ, interleukin 4, and progesterone induced blocking factor were determined using real-time PCR. The expression of interleukin 4 and progesterone induced blocking factor was significantly higher in WC1+ γδ T cells from gravid cows. In addition, interleukin 4 expression in WC1+ γδ T cells from gravid cows was significantly higher than that in CD4+ and CD8+ T cells. This study describes for the first time the important role of WC1+ γδ T cells during the preimplantation period in cows.

Keywords

CD4, CD8, IFN-γ, IL-4, PIBF, WC1.

References

15 live references