Acta Vet. Brno 2014, 83: 55-60

Mechanical testing of newly developed biomaterial designed for intra-articular reinforcement of partially ruptured cranial cruciate ligament: ex vivo pig model

Petra Fedorová1, Robert Srnec2, Jan Pěnčík3, Pavel Schmid3, Evžen Amler4,5, Lucie Urbanová1, Alois Nečas2

1University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Medicine, Small Animal Clinic, Department of Surgery and Orthopaedics, Brno, Czech Republic
2CEITEC - Central European Institute of Technology, Brno, Czech Republic
3Brno University of Technology, Faculty of Civil Engineering, Institute of Building Testing, Brno, Czech Republic
4Academy of Sciences of the Czech Republic, v.v.i., Laboratory of Tissue Engineering, Institute of Experimental Medicine, Prague, Czech Republic
5Charles University in Prague, 2nd Faculty of Medicine, Institute of Biophysics, Prague, Czech Republic

The study deals with mechanical testing of newly developed material polyethylene terephtalate coated with polycaprolactone nanofibers in combination with biodagradable Hexalon ACL/PCL screws as a new possibility of intra-articular reinforcement of partially ruptured cranial cruciate ligament. Four groups of ex vivo models of pig stifle joints were prepared and tested: a model with intact CCL (group 1), a model with partial CCL rupture (group 2), a model with CCL rupture stabilized with 7 mm Mersilene® strip (group 3), and a model with CCL rupture stabilized with 5 mm PET/PCL biomaterial strip (group 4). The models were loaded in the standing angle of 100° and the maximum load (N) and the shift (mm) were monitored. The mean maximum peak power and the shift were 1266.0 ± 146.9 N and 13.7 ± 2.5 mm for group 1, and 1164.7 ± 228.2 N and 1 6.8 ± 3.3 mm for group 2, respectively. In all cases after reaching the maximum load, a tibial fracture occurred but never a CCL rupture. In groups 3 and 4, the initial fixation failure occurred in the mean values of 375.7 ± 81.5 and 360.4 ± 52.0 N, respectively, and with a bigger shift of 52.3 ± 11.9 mm and 39.4 ± 14.6 mm, respectively, compared to group 1. A critical point of failure was the anchoring in the bone. It can be concluded that the PET/PCL substitute in the ex vivo model has mechanically comparable properties with the clinically used Mersilene®, and based on its proven ability to carry stem cells it could be appropriate for partially ruptured CCL protection.


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