PCR detection of <i>Bartonella</i> spp. in the dog

Konvalinová, Jarmila; Svobodová, Vlasta; Molinková, Dobromila; Svoboda, Miroslav

doi:10.2754/avb201483020079

Acta Veterinaria > Archive > 2014, Vol. 83 > Issue 2 > PCR detection of Bartonella spp.…

Acta Vet. Brno 2014, 83: 79-82

https://doi.org/10.2754/avb201483020079

PCR detection of Bartonella spp. in the dog

Jarmila Konvalinová¹, Vlasta Svobodová², Dobromila Molinková³, Miroslav Svoboda¹

¹University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Medicine, Small Animal Clinic, Brno, Czech Republic
²University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Medicine, Department of Pathological Morphology and Parasitology, Brno, Czech Republic
³University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Medicine, Department of Infectious Diseases and Microbiology, Brno, Czech Republic

Our study aimed at using PCR to identify the incidence of Bartonella spp. in blood of dogs. Altogether 286 dogs of 92 breeds aged 3 month to 17 years were tested from October 2008 to December 2009. Healthy dogs as well as dogs with various clinical symptoms of disease were included in the group. Samples were tested by polymerase chain reaction (PCR) specific for the presence of Bartonella spp. Following the DNA examination in 286 dogs by PCR and subsequent sequencing, two samples were identified as Bartonella henselae (0.7%). Other species of Bartonella were not found. It was the first time in the Czech Republic when incidence of Bartonella spp. was determined in dogs.