Acta Vet. Brno 2014, 83: 313-319

Modified hypoosmotic swelling test for the assessment of boar and bull sperm sensitivity to cryopreservation

Petra Přinosilová, Věra Kopecká, Jaroslava Hlavicová, Monika Kunetková

Veterinary Research Institute, Brno, Czech Republic


1. Amann RP 1989: Can the fertility potential of a seminal sample be predicted accurately? J Androl 10: 89-98 <>
2. Casas I, Sancho S, Briz M, Pinart E, Bussalleu E, Yeste M, Bonet S 2010: Fertility after post-cervical artificial insemination with cryopreserved sperm from boar ejaculates of good and poor freezability. Anim Reprod Sci 118: 69-76 <>
3. Chan PJ, Tredway DR, Corselli J, Pang SC, Su BC 1991: Combined supravital staining and hypoosmotic swelling test. Hum Reprod 6: 1115-1118 <>
4. Correa JR, Zavos PM 1994: The hypoosmotic swelling test: its employment as an assay to evaluate the functional integrity of the frozen-thawed bovine sperm membrane. Theriogenology 42: 351-360 <>
5. Eilts BE 2005: Theoretical aspects of canine semen cryopreservation. Theriogenology 64: 692-697 <>
6. Hammerstedt RH 1979: Characterization of sperm surface using physical techniques. In: Fawcett DW and Bedford JM (Eds). The spermatozoon: Maturation, Motility, Surface Properties and Comparative Aspects. Urban and Schwarzenberg, Baltimore, USA, pp. 205-216
7. Holt WV, Medrano A, Thurston LM, Watson PF 2005: The significance of cooling rates and animal variability for boar sperm cryopreservation: insights from the cryomicroscope. Theriogenology 63: 370-382 <>
8. Jeyendran RS, Van der Ven HH, Perez-Pelaez M, Crabo BG, Zaneveld LJD 1984: Development of an assay the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J Reprod Fert 70: 219-228 <>
9. Kruger TF, Menkveld R, Stander FSH, Lombard CJ, Van der Merwe JP, Van Zyl JA, Smith K 1986: Sperm morphologic features as a prognostic factor in vitro fertilization outcome. Fertil Steril 46: 1118-1123 <>
10. Parks JE, Lynch DV 1992: Lipid composition and thermotropic phase behavior of boar, bull, stallion, and rooster sperm membranes. Cryobiology 29: 255-266 <>
11. Peña FJ, Saravia F, Johannisson A, Walgren M, Rodríguez-Martínez H 2005: A new and simple method to evaluate early membrane changes in frozen-thawed boar spermatozoa. Int J Androl 28: 107-114 <>
12. Peña FJ, Saravia F, Johannisson A, Wallgren M, Rodríguez-Martínez H 2007: Detection of early changes in sperm membrane integrity pre-freezing can estimate post-thaw quality of boar spermatozoa. Anim Reprod Sci 97: 74-83 <>
13. Pérez-Liano B, Lorenzo JL, Yenes P, Trejo A, Garcia-Casado P 2001: A short hypoosmotic swelling test for the prediction of boar sperm fertility. Theriogenology 56: 387-98 <>
14. Roca J, Hernández M, Carvajal G, Vázquez JM, Martinez EA 2006: Factors influencing boar sperm cryosurvival. J Anima Sci 84: 2692-2699 <>
15. Rodriguez-Martinez H 2003: Laboratory semen assessment and prediction of fertility: still utopia? Reprod Domest Anim 38: 312-318 <>
16. Rota A, Penzo N, Vincenti L, Mantovani R 1999: Hypoosmotic swelling (HOS) as a screening assay for testing in vitro fertility of bovine spermatozoa. Theriogenology 53: 1415-1420 <>
17. Sion B, Janny L, Boucher D, Grizard G 2004: Annexin V binding to plasma membrane predicts the quality of human cryopreserved spermatozoa. Int J Androl 27: 108-114 <>
18. Tartaglione CM, Ritta MN 2004: Prognostic value of spermatological parameters as predictors of in vitro fertility of frozen-thawed bull semen. Theriogenology 62: 1245-1252 <>
19. Thurston LM, Watson PF, Holt WV 1999: Sources of variation in boar spermatozoa fertility following cryopreservation. Cryobiology 39: 355
20. Vazquez JM, Martinez EA, Martinez P, Garcia-Artiga C, Roca J 1997: Hypoosmotic swelling of boar spermatozoa compared to other methods for analysing the sperm membrane. Theriogenology 4: 913-922 <>
21. Veznik Z, Matouskova O, Svecova D, Zajicova A 2001: The use of the computer technology for the evaluation of the strict morphological sperm analysis. Vet Med-Czech 46: 35-40 <>
22. Věžník Z, Přinosilová P, Zajícová A, Kunetková M, Hlavicová J 2010: Evaluation of sperm membrane resistance in boars by modification of HOS test (in Czech).Veterinářství 60: 695-697
23. Westendorf P, Richter L, Treu H 1975: Deep freezing of boar sperma. Laboratory and insemination results using the Hülsenberger paillet method (in German). Dtsch Tierarztl Wochenschr 82: 261-267
24. World Health Organization 2010: WHO Laboratory Manual for the Examination of Human Semen. 5th ed. WHO Press, Geneva, Switzerland, 271 p.s
front cover
  • ISSN 0001-7213 (printed)
  • ISSN 1801-7576 (electronic)

Current issue

Indexed in DOAJ