Acta Vet. Brno 2014, 83: 411-416

Ketone bodies in blood of dairy cows: Prevalence and monitoring of subclinical ketosis

Marek Krempaský, Iveta Maskaľová, Lukáš Bujňák, Vladimír Vajda

University of Veterinary Medicine and Pharmacy in Košice, Department of Nutrition, Dietetics and Animal Breeding, Košice, Slovak Republic


1. Adewuyi A, Gruys E, Van Eerdenburg F 2005: Non esterified fatty acids (NEFA) in dairy cattle. A review. Vet Q 27: 117-126 <>
2. Chapinal N, Carson M, Duffield T, Capel M, Godden S, Overton M, Santos J, Le Blanc S 2011: The association of serum metabolites with clinical disease during the transition period. J Dairy Sci 94: 4897-4903 <>
3. Dohoo I, Wayne Martin SW, Meek A, Sandals W 1983: Disease, production and culling in Holstein-Friesian cows I. Prev Vet Med 1: 321-334 <>
4. Dolník V, Boček P 1981: Determination of pyruvate, lactate, acetoacetate, and 3-hydroxybutyrate in serum by capillary isotachophoresis. J Chrom 225: 455-458 <>
5. Duffield T, Sandals F, Leslie K, Lissemore K, McBride B, Lumsden J, Dick P, Bagg R 1998: Efficacy of monensin for the prevention of subclinical ketosis in lactating dairy cows. J Dairy Sci 81: 2866-2873 <>
6. Duffield T 2000: Subclinical ketosis in lactating dairy cattle. Vet Clin North Am Food Anim Pract 16: 231-253 <>
7. Duffield T, Lissemore K, McBride B, Leslie K 2009: Impact of hyperketonemia in early lactation dairy cows on health and production. J Dairy Sci 92: 571-580 <>
8. Duncombe 1964: The colorimetric microdetermination of non-esterified fatty acids in plasma. Clin Chim Acta 9: 122-125 <>
9. Enjalbert F, Nicot MC, Bayourthe C, Moncoulon R 2001: Ketone bodies in milk and blood of dairy cows: relationship between concentrations and utilization for detection of subclinical ketosis. J Dairy Sci 84: 583-589 <>
10. Geishauser T, Leslie K, Kelton D, Duffield T 1998: Evaluation of five cowside tests for use with milk to detect subclinical ketosis in dairy cows. J Dairy Sci 81: 438-443 <>
11. Iwersen M, Falkenberg U, Voigtsberger R, Forderung D, Heuwieser W 2009: Evaluation of an electronic cowside test to detect subclinical ketosis in dairy cows. J Dairy Sci 92: 2618-2624 <>
12. McArt J, Nydam D, Ospina P, Oetzel G 2011: A field trial on the effect of propylene glycol on milk yield and resolution of ketosis in fresh cows diagnosed with subclinical ketosis. J Dairy Sci 94: 6011-6020 <>
13. National Research Council 2001. Nutrient Requirements of Dairy Cattle, 7th rev. ed. Washington, D.C., National Academy Press
14. Nogalski Z, Wroński M, Lewandowska B, Pogorzelska P 2012: Changes in the blood indicators and body condition of high yielding Holstein cows with retained placenta and ketosis. Acta Vet Brno 81: 359-364 <>
15. Oetzel G 2013: Understanding the impact of subclinical ketosis. 24th Ruminant Nutrition Symposium. Available at: Last modified February 2013. Accessed February, 2013
16. Rollin E, Berghaus R, Rapnicki P, Godden S, Overton M 2010: The effect of injectable butaphosphan and cyano-cobalamin on postpartum serum β-hydroxybutyrate, calcium, and phosphorus concentrations in dairy cattle. J Dairy Sci 93: 978-987 <>
17. Suthar VS, Canelas-Raposo J, Deniz A, Heuwieser W 2013: Prevalence of subclinical ketosis and relationships with postpartum diseases in European dairy cows. J Dairy Sci 96: 2925-2938 <>
18. Williamson H, Mellanby J, Krebs H 1962: Enzymic determination of D(-)-β-hydroxybutyric acid and acetoacetic acid in blood. J Biochem 82: 90-96 <>
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  • ISSN 0001-7213 (printed)
  • ISSN 1801-7576 (electronic)

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