Activity of Na+/K+-activated Mg2+-dependent ATP-hydrolase in the cell-free extracts of the sulfate-reducing bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9

Kushkevych, Ivan; Fafula, Roman; Parák, Tomáš; Bartoš, Milan

doi:10.2754/avb201585010003

Acta Veterinaria > Archive > 2015, Vol. 84 > Issue 1 > Activity of…

Acta Vet. Brno 2015, 84: 3-12

https://doi.org/10.2754/avb201585010003

Activity of Na⁺/K⁺-activated Mg²⁺-dependent ATP-hydrolase in the cell-free extracts of the sulfate-reducing bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9

Ivan Kushkevych¹, Roman Fafula², Tomáš Parák¹, Milan Bartoš¹

¹University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Pharmacy, Department of Human Pharmacology and Toxicology, Brno, Czech Republic
²Danylo Halytsky Lviv National Medical University, Faculty of Pharmacy, Lviv, Ukraine

Received March 28, 2014
Accepted October 22, 2014

The aim of our work was to study Na⁺/K⁺-activated Mg²⁺-dependent ATPase activity in cell-free extracts of the sulfate-reducing bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9 isolated from the human large intestine, and to carry out the kinetic analysis of the enzyme reaction. The maximum ATPase activity for both bacterial strains at +35 ºC was determined. The highest activities of the studied enzyme in the cell-free extracts of D. piger Vib-7 at pH 7.0 and Desulfomicrobium sp. Rod-9 at pH 6.5 were measured. Based on experimental data, the analysis of kinetic properties of the ATP-hydrolase reaction by the studied bacteria was carried out. The enzyme activity, initial (instantaneous) reaction rate (V₀) and maximum rate of the ATPase reaction (V_max) was significantly higher in D. piger Vib-7 cells than in Desulfomicrobium sp. Rod-9. Michaelis constants (K_m) of the enzyme reaction for both bacterial strains were determined.