Acta Vet. Brno 2015, 84: 97-104

https://doi.org/10.2754/avb201584020097

Isolation and identification of Dichelobacter nodosus and Fusobacterium necrophorum using the polymerase chain reaction method in sheep with footrot

Ediz Kagan Ozgen1, Seyda Cengiz2, Mustafa Ulucan1, Zafer Okumus3, Asli Kortel1, Hüseyin Erdem1, Huseyin Gurkan Sarac1

1Erzurum Veterinary Control Institute, Ministry of Food, Agriculture and Livestock, Erzurum, Turkey
2Atatürk University, Faculty of Veterinary Medicine, Department of Microbiology, Erzurum, Turkey
3Atatürk University, Faculty of Veterinary Medicine, Department of Surgery, Erzurum, Turkey

Received June 25, 2014
Accepted February 25, 2015

References

1. Allworth B 2004: Footrot. In: Sheep medicine – Proceedings 355 Post Graduate Foundation in Veterinary Science University of Sydney, Sydney, pp. 227-253
2. Bennett G, HickfordJ, Sedcole R, Zhou H 2009a: Dichelobacter nodosus, Fusobacterium necrophorum and the epidemiology of footrot. Anaerobe 15: 173-176 <https://doi.org/10.1016/j.anaerobe.2009.02.002>
3. Bennett G, Hickford J, Zhou H, Laporte J, Gibbs J 2009b: Detection of Fusobacterium necrophorum and Dichelobacter nodosus in lame cattle on dairy farms in New Zealand. Res Vet Sci 87: 413-5. <https://doi.org/10.1016/j.rvsc.2009.04.001>
4. Bennett G, Loenen AV, Zhou H, Sedcole R, Hickford J 2009c: The detection of Dichelobacter nodosus and Fusobacterium necrophorum from footrot lesions in New Zealand goats. Anaerobe 15: 177 <https://doi.org/10.1016/j.anaerobe.2009.02.003>
5. Bhat MA, Wani SA, Hussain I, Magray SN, Muzafar M 2012: Identification of two new serotypes within serogroup B of Dichelobacter nodosus. Anaerobe 18: 91-95 <https://doi.org/10.1016/j.anaerobe.2011.12.010>
6. Cagatay IF, Hickford JGH 2006: Characterization of footrot bacteria Dichelobacter nodosus using PCR amplification and DNA sequence analysis. Turk J Vet Anim Sci 30: 53-59
7. Dhungyel O, Schiller N, Eppleston J, Lehmann D, Nilon P, Ewers A, Whittington R 2013: Outbreak-specific monovalent/bivalent vaccination to control and eradicate virulent ovine footrot. Vaccine 31: 1701-1706 <https://doi.org/10.1016/j.vaccine.2013.01.043>
8. Garrity GM, Bell JA, Lilburn T 2005: Dichelobacter spp., Cardiobacteriales, In: George GM, Staley JT, Boone DR, Brenner DJ, De Vos P, Garrity GM, Goodfellow M, Krieg NR, Rainey FA, Karl-Heinz Schleifer KH (Eds): Bergey’s Manual of Systematic Bacteriology. Springer, New York, pp. 126-131
9. Ghimire SC, Egertonb JR, Dhungyel OP, Joshia HD 1998: Identification and characterization of serogroup M among Nepalese isolates of Dichelobacter nodosus, the transmitting agent of footrot in small ruminants. Vet Microbiol 62: 217-233 <https://doi.org/10.1016/S0378-1135(98)00206-5>
10. Lee SS, Ahn BH, Kim HS, Kim CH, Cheng KJ, Ha JK 2003: Effects of non-ionic surfactants on enzyme distributions of rumen contents, anaerobic growth of rumen microbes, rumen fermentation characteristics and performances of lactating cows. Asian-Aust J Anim Sci 16: 104-115 <https://doi.org/10.5713/ajas.2003.104>
11. Moore LJ, Wassink GJ, Green LE, Grogono-Thomas R 2005: The detection and characterization of Dichelobacter nodosus from cases of ovine footrot in England and Wales. Vet Microbiol 108: 57-67 <https://doi.org/10.1016/j.vetmic.2005.01.029>
12. Morgenstein AA, Citron DM, Finegold SM 1981: New medium selective for Fusobacterium species and differential for Fusobacterium necrophorum. J Clin Microbiol 13: 666-669
13. Nitzan Y, Wexler HM, Finegold SM 1994: Inactivation of anaerobic bacteria by various photosensitized porphyrins or by hemin. Curr Microbiol 29: 125-131 <https://doi.org/10.1007/BF01570752>
14. Noel AR, Bowker KE, MacGowan AP 2005: Pharmacodynamics of moxiloxacin against anaerobes studied in an in vitro pharmacokinetic model. Antimicrob Agents Chemother 49: 4234-4239 <https://doi.org/10.1128/AAC.49.10.4234-4239.2005>
15. Petrov KK, Dicks LMT 2013: Fusobacterium necrophorum, and not Dichelobacter nodosus, is associated with equine hoof thrush. Vet Microbiol 161: 350-352 <https://doi.org/10.1016/j.vetmic.2012.07.037>
16. Quinn PJ, Carter ME, Markey BK, Carter GR 2004: Non-spore-forming anaerobic bacteria. In Ouinn PJ (Ed.): Clinical Veterinary Microbiology. Mosby Press, London, pp. 184-190
17. Raadsma HW, Egerton JR 2013: A review of footrot in sheep: aetiology, risk factors and control methods. Livest Sci 156: 106-114 <https://doi.org/10.1016/j.livsci.2013.06.009>
18. Rather MA, Wani SA, Hussain I, Bhat MA, Kabli ZA, Magray SN 2011: Determination of prevalence and economic impact of ovine footrot in central Kashmir India with isolation and molecular characterization of Dichelobacter nodosus. Anaerobe 17: 73-77 <https://doi.org/10.1016/j.anaerobe.2011.02.003>
19. Rood JI, Howarth PA, Haring V, Billington SJ, Yong WK, Liu D, Palmer MA, Pitman DR, Links I, Stewart DJ, Vaughan JA 1996: Comparison of gene probe and conventional methods for the differentiation of ovine footrot isolates of Dichelobacter nodosus. Vet Microbiol 52: 127-141 <https://doi.org/10.1016/0378-1135(96)00054-5>
20. Sagliyan A 2003: Clinical evaluation of sheep foot diseases observed in Elazığ region. Fırat Univ Vet J Health Sci 17: 39-44
21. Sagliyan A, Gunay C, Koparir M 2003: The role of copper and zinc in the etiology of foot-rot of sheep in Elazığ region. Turk J Vet Surg 9: 11-16
22. Sertkaya H, Sindak N 2004: Incidence and treatment with two different drug combinations of sheep’s footrot in Birecik District of Şanlıurfa and its villages. Turk J Vet Surg 10: 48-54
23. Stewart DJ, Claxton PD 1982: Ovine footrot clinical diagnosis and bacteriology. In: Corner, LA, Bagust TJ (Eds): Australian Standard Diagnostic Techniques for Animal Diseases, CSIRO Information Services, Melbourne, pp. 1-27
24. Tadepalli S, Narayanan SK, Stewart GC, Chengappa MM, Nagaraja TG 2009: Fusobacterium necrophorum: a ruminal bacterium that invades liver to cause abscesses in cattle. Anaerobe 15: 36-43 <https://doi.org/10.1016/j.anaerobe.2008.05.005>
25. Timoney JF, Gillespie JH, Scott FW, Barlough JE 1988: The Genera Fusobacterium and Bacteroides In: Timoney JF, Gillespie JA, Scott FW, Barlough JE (Eds): Hagan and Bruner’s Microbiology and Infectious Diseases of Domestic Animals. Cornell University Press. New York. pp. 161-170
26. Zhou H, Bennett G, Hickford J 2009: Variation in Fusobacterium necrophorum strains present on the hooves of footrot infected sheep, goats and cattle. Vet Microbiol 135: 363-267 <https://doi.org/10.1016/j.vetmic.2008.09.084>
27. Zhou H, Lottner S, Ganter M, Hickford JGH 2010: Identification of two new Dichelobacter nodosus strains in Germany. The Vet J 184: 115-117 <https://doi.org/10.1016/j.tvjl.2009.02.003>
front cover
  • ISSN 0001-7213 (printed)
  • ISSN 1801-7576 (electronic)

Current issue

Archive