Acta Vet. Brno 2015, 84: 125-131

Influence of selected factors on bovine spermatozoa cold shock resistance

Luděk Stádník1, Radko Rajmon2, Jan Beran1, Ondřej Šimoník2, Martina Doležalová1, Jiří Šichtař2, Roman Stupka1, Petra Folková2

1Czech University of Life Sciences Prague, Faculty of Agrobiology, Department of Animal Husbandry, Prague, Czech Republic
2Czech University of Life Sciences Prague, Faculty of Agrobiology, Department of Veterinary Sciences, Prague, Czech Republic

Received October 22, 2014
Accepted February 25, 2015

The objectives of this study were to determine the effects of sire, extender, and addition of Low Density Lipoprotein (LDL) to extenders used on the percentage rate of spermatozoa survival after cold shock. Two groups of extenders were compared: without LDL addition (control variants) and LDL enriched (experimental variants). Three extenders were used: AndroMed®, Bioxcell®, and Triladyl®. Experimental variants included 4–8% LDL addition into the AndroMed® and Bioxcell® extenders, and 6–10% LDL addition into the Triladyl® extender. In total, 12 samples of fresh semen were collected from 4 bulls during a period of 8 weeks. Bovine spermatozoa cold shock resistance (1 ± 1 °C, 10 min) was evaluated by the percentage rate of live sperm using eosin-nigrosine staining immediately and after heat incubation (37 ± 1 °C, 120 min). The results showed the effect of sire as important and individual differences between selected sires in their sperm resistance against cold shock were confirmed. AndroMed® and Bioxcell® were found to be providing better protection of bull semen to cold shock compared to Triladyl® due to lower decline of live sperm proportion. Our results detected a positive effect of LDL addition on sperm resistance against cold shock, especially on lower decrease of live sperm percentage rate after 120 min of the heat test (P < 0.05). Further studies are needed to assess the optimal concentration of LDL in various kinds of extenders as well to state ideal time and temperature conditions for ensuring LDL reaction with sperm.


The work was funded by “S” grant of MŠMT ČR and project NAZV QJ1210109.


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