Acta Vet. Brno 2015, 84: 327-336

Polymorphic effects of FABP4 and SCD genes on intramuscular fatty acid profiles in longissimus muscle from two cattle breeds

Radka Dujková1, Yuvaraj Ranganathan1, Aleš Dufek1, Jan Macák1, Jiří Bezdíček2

1Agroresearch Rapotín Ltd., Vikýřovice, Czech Republic
2Palacký University, Faculty of Science, Department of Zoology, Olomouc, Czech Republic

Received December 2, 2014
Accepted September 23, 2015

The aim of this study was to evaluate the polymorphic effects of two single nucleotide polymorphisms (SNPs) of fatty acid binding protein (FABP4) and stearoyl-CoA desaturase (SCD) genes on intramuscular fatty acid profiles in the longissimus muscle in two cattle breeds. Two previously reported SNPs of bovine FABP4 (7516G>C) and SCD (878C>T) were in turn assessed for their associations with intramuscular fatty acid profiles from the upper sirloin cuts of Aberdeen Angus and Blonde d’Aquitaine cattle. In total, 33 animals were genotyped using PCR-RFLP. Intramuscular fatty acid composition was evaluated using two complementary statistical approaches: a classical univariate regression model and a multivariate approach using a combination of Principal Component Analysis and Random Forests. Significant effect of FABP4 SNP genotypes was found for several fatty acids including C15:0, C17:0, C18:0, C14:1, C17:1, C18:2n6, C20:4n6, C20:5n3, C22:5n3, total n-3, n-6 and total SFA (P < 0.05). These results suggest that FABP4 is a potential candidate gene affecting fatty acid composition in beef cattle.


The authors acknowledge the funding support by the Ministry of Education, Youth and Sports (under the project titled, “Development of human resources in food analysis using gas chromatography”, Reg. No. CZ.1.07/2.3.00/30.0008, Ministry of Agriculture of the Czech Republic through institutional support by decision RO1214 of February 27th 2014, Technology Agency of the Czech Republic (project TA01010153).


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