Acta Vet. Brno 2016, 85: 157-164

Seasonal dynamics and possible development of total count of microorganisms in sheep’s milk

Marcela Klimešová1, Martin Tomáška2, Margita Hofericová2, Oto Hanuš1, Lenka Vorlová3, Ludmila Nejeschlebová1, Hana Nejeschlebová3, Lucie Hasoňová4, Jaroslav Kopecký1, Eva Vondrušková1

1Dairy Research Institute, Prague, Czech Republic
2Dairy Research Institute, Žilina, Slovakia
3University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Hygiene and Ecology, Department of Milk Hygiene and Technology, Czech Republic
4University of South Bohemia in České Budějovice, Faculty of Agriculture Department of Agricultural Products Quality, Czech Republic

Received September 22, 2015
Accepted May 2, 2016

The work is focused on the evaluation of seasonal dynamics of the total count of microorganisms in sheep’s milk and on the proposal of developing cut-off values for standard limitation for the next fifteen years for hygienic safety support. The total count of microorganisms was measured between years 2012–2014 (n = 4,746). The results were statistically evaluated using medians, geometric means, arithmetic means, means of log values (log10) and standard deviations for the cut-off limit determination. This model was patterned on the maximum of total count of microorganisms in the tested percentiles 95 (statistical conventional interval), 91, 90, 80 and 70% and on the result of medians (from 109 to 148 × 103 cfu/ml). These cut-off limits were divided into three classes (I to III) of standard quality, and the model of dynamics for their gradual implementation time was created as follows: initiation period (class I = ≤ 800; II = from 801 to 1,300; III = 1,301 to 4,000; non-standard = > 4,000 × 103 cfu/ml); second period (class I = ≤ 550; II = 551 to 800; III = 801 to 1,300; non-standard = > 1,300 × 103 cfu/ml); third period (standard class = ≤ 800 × 103 cfu/ml); fourth period (standard class = ≤ 550 × 103 cfu/ml); and the last legislative period (≤ 300 × 103 cfu/ml as a real hygienic limit). The limit for milk without heat treatment corresponds to the real value of the median 200 × 103 cfu/ml. The work is important for the procedures in the development of methods for the control and safety of raw food material.


This study was supported by the Slovak Research and Development Agency on the basis of Treaty APVV-0357-12 and by the Ministry of Agriculture of the Czech Republic with the project NAZV KUS QJ1230044 and by the Ministry of Agriculture of the Czech Republic (decision No. R01416).


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