Prevalence of respiratory pathogens detected in dogs with kennel cough in Poland

Kaczorek, Edyta; Schulz, Patrycja; Małaczewska, Joanna; Wójcik, Roman; Siwicki, Andrzej Krzysztof; Stopyra, Artur; Lew-Kojrys, Sylwia; Pomianowski, Andrzej; Hryniewicka, Karolina; Mikulska-Skupień, Elżbieta

doi:10.2754/avb201685040329

Acta Veterinaria > Archive > 2016, Vol. 85 > Issue 4 > Prevalence of respiratory pathogens…

Acta Vet. Brno 2016, 85: 329-336

https://doi.org/10.2754/avb201685040329

Prevalence of respiratory pathogens detected in dogs with kennel cough in Poland

Edyta Kaczorek¹, Patrycja Schulz¹, Joanna Małaczewska¹, Roman Wójcik¹, Andrzej Krzysztof Siwicki¹, Artur Stopyra², Sylwia Lew-Kojrys², Andrzej Pomianowski², Karolina Hryniewicka², Elżbieta Mikulska-Skupień³

¹University of Warmia and Mazury in Olsztyn, Faculty of Veterinary Medicine, Department of Microbiology and Clinical Immunology, Olsztyn, Poland
²University of Warmia and Mazury in Olsztyn, Faculty of Veterinary Medicine, Department of Internal Diseases with Clinic, Olsztyn, Poland
³University of Warmia and Mazury in Olsztyn, Faculty of Veterinary Medicine, Department of Epizootiology, Olsztyn, Poland

Received December 17, 2015
Accepted December 2, 2016

Kennel cough is a multifactorial disease occurring all over the world; however, its epidemiology is still not fully understood. To the authors’ knowledge, no studies monitoring the occurrence of infectious agents responsible for kennel cough have been carried out in Poland. Therefore, the objective of our study was to determine which of the four pathogens most frequently isolated in other countries are predominant in north-eastern Poland. Swabs from the upper respiratory tract and tracheal lavage fluids from dogs (n = 40) exhibiting symptoms of this disease were analysed. Canine herpesvirus, canine parainfluenza virus, canine adenovirus type 2 and Bordetella bronchiseptica were identified by polymerase chain reaction. At least one of the above-listed infectious agents was found in all dogs. The predominant pathogen within the area under our study, both in mono- and co-infections, was canine herpesvirus (32/40), whereas canine adenovirus type 2 occurred least frequently (4/40). The effectiveness of detection of selected pathogens from both types of study material was also compared. Tracheal lavage fluid was more suitable for the isolation of canine herpes virus, canine parainfluenza virus, and Bordetella bronchiseptica. Swabs from the upper respiratory tract were more suitable for the isolation of canine adenovirus type 2.