Acta Vet. Brno 2017, 86: 3-10

Occurrence of Staphylococcus aureus in cattle, sheep, goat, and pig rearing in the Czech Republic

Marcela Klimešová1, Ivan Manga2, Ludmila Nejeschlebová1, Jiří Horáček2, Antonín Ponížil2, Eva Vondrušková1

1Dairy Research Institute, Prague, Czech Republic
2Agritec Plant Research, Šumperk, Czech Republic

Received June 22, 2016
Accepted March 3, 2017

The study focused on the prevalence of Staphylococcus aureus and resistant strains in livestock. In this study, 114 different samples from three cattle farms (84 from two farms of dairy cows and 30 from one farm of suckler cows), 132 samples from one sheep farm, 120 samples from one goat farm, and 82 samples from three pig farms were examined. Strains identified as Staphylococcus aureus were further analysed by the polymerase chain reaction method for detection of the mecA gene and for confirmation of the sequence type 398. Positive incidence of Staphylococcus aureus was confirmed in farms of suckler cows, sheep, goats and pigs. The incidence of methicilin-resistant Staphylococcus aureus was confirmed at a goat farm, with all strains belonging to the sequence type 398. Repetitive element palindromic-polymerase chain reaction analysis was performed to compare the relatedness of selected human and animal S. aureus strains at the goat and sheep farms. The obtained data from repetitive element-polymerase chain reaction analysis showed significant clonal similarity among the tested isolates and indicated the possibility of mutual transmission between animals or animal and human and possible transfer in the food chain.


This work was supported by the Ministry of Agriculture of the Czech Republic with the project NAZV KUS QJ1210284 and QJ1510216 and decision No. RO1416.


36 live references