Acta Vet. Brno 2017, 86: 59-65

Investigation of negative energy balance and postpartum anoestrus in an intensive dairy farm from the Chinese province of Heilongjiang

Ziling Fan1, Shi Shu1, Chuchu Xu1, Changsheng Li1, Xinhuan Xiao1, Cheng Xia1,2, Gang Wang1, Hongyou Zhang1, Chuang Xu1, Wei Yang1

1Heilongjiang Bayi Agricultural University, Department of College of Animal Science and Veterinary Medicine, Daqing, China
2Northeast Agricultural University, Department of Synergetic Innovation Centre of Food Safety and Nutrition, Harbin, China

Received July 28, 2016
Accepted March 3, 2017

The aim of this investigation was to determine the relationship between postpartum anoestrus and negative energy balance in an intensive dairy farm from the Heilongjiang Province, China. At 14 to 21 d after parturition, 100 cows were randomly selected and their plasma indices, including β-hydroxybutyric acid, non-esterified fatty acid, and glucose were measured. Cows were assigned to a positive energy balance group (n = 37) and a negative energy balance group (n = 36) based on their β-hydroxybutyric acid concentrations (> 1.20 mmol/l). The two groups of cows were examined by B-mode ultrasonography and rectal examination from 60 to 90 d after parturition to identify the ovarian status of oestrous and anoestrous animals. The incidences of negative energy balance and positive energy balance were 49 and 57%, respectively, from14 to 21 d after parturition. From 60 to 90 d after parturition, 94.4% of the negative energy balance group were in anoestrus and 5.6% were in oestrus, while 62.2% of the positive energy balance group were in anoestrus and 37.8% were in oestrus. Furthermore, the proportion of inactive ovaries in the negative energy balance group was 61.8%. In conclusion, the negative energy balance is an important factor causing inactive ovaries in high-yielding dairy cows.


This work was supported by: the National Science Foundation Committee under Grant no. 31372488; the National Science and Technology Support Program under Grant no. 2013BAD21B01; the China Spark Program under Grant no. 2012GA670001; the Program for New Century Excellent Talents of Heilongjiang Province under Grant no. 1252-NCET-003; and the Natural Science Foundation of Heilongjiang Province of China under Grant no. C2016041.


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