Acta Vet. Brno 2017, 86: 175-181

The determination of minimum inhibitory concentrations of selected antimicrobials for porcine Haemophilus parasuis isolates from the Czech Republic

Kateřina Nedbalcová1, Monika Zouharová1, Daniel Šperling2

1Veterinary Research Institute, Brno, Czech Republic
2Ceva Santé Animale S.A., Libourne, France

Received February 7, 2017
Accepted May 31, 2017

Haemophilus parasuis isolates obtained from pigs in the Czech Republic were tested for their susceptibility to amoxicillin, penicillin, ceftiofur, enrofloxacin, tetracycline, and tulathromycin by determination of minimum inhibitory concentrations using the broth microdilution method. The H. parasuis isolates were mostly susceptible to majority of tested antimicrobials (amoxicillin 90%, penicillin 73.3%, enrofloxacin 83.3%, and tulathromycin 83.3%). All isolates were susceptible to ceftiofur. On the other hand, no isolate was susceptible to tetracycline, 30% of tested isolates were intermediately susceptible, and 70% were resistant. These findings indicate that tested antimicrobials with the exception of tetracycline should be the preferred option used for the treatment of infection caused by H. parasuis but due to the potential transmission of resistance from animals to humans, the use of ceftiofur is considered as a last resort option in antimicrobial treatment of animals.


This work was supported by the Ministry of Agriculture of the Czech Republic (Project No. RO 0516) and the Ministry of Education, Youth and Sports of the Czech Republic (Project No. LO 1218 NPU I).


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