Acta Vet. Brno 2017, 86: 207-212

https://doi.org/10.2754/avb201786030207

Diagnostic efficacy of molecular assays for the viral haemorrhagic septicaemia virus isolates from the Czech Republic

Ľubomír Pojezdal1,2, Dagmar Pokorová2, Stanislava Reschová2, Miroslava Palíková1, Monika Vícenová2, Tomáš Veselý2, Stanislav Navrátil1

1University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Hygiene and Ecology, Department of Ecology and Diseases of Game, Fish and Bees, Brno, Czech Republic
2Veterinary Research Institute, Department of Virology, National Reference Laboratory for Viral Diseases of Fish, Brno, Czech Republic

Received May 26, 2017
Accepted October 2, 2017

References

1. Commission Decision (EU) 2001/183/EC of 22 February 2001 laying down the sampling plans and diagnostic methods for the detection and confirmation of certain fish diseases and repealing Decision 92/532/EEC
2. Commission Implementing Decision (EU) 2015/1554 of 11 September 2015 laying down rules for the application of Directive 2006/88/EC as regards requirements for surveillance and diagnostic methods
3. Einer-Jensen K, Ahrens P, Forsberg R, Lorenzen N 2004: Evolution of the fish rhabdovirus viral haemorrhagic septicaemia virus. J Gen Virol 85: 1167-1179 <https://doi.org/10.1099/vir.0.79820-0>
4. Garver KA, Hawley LM, McClure CA, Schroeder T, Aldous S, Doing F, Snow M, Edes S, Baynes C, Richard J 2011: Development and validation of a reverse transcription quantitative PCR for universal detection of viral hemorrhagic septicemia virus. Dis Aquat Org 95: 97-112 <https://doi.org/10.3354/dao02344>
5. Gilad O, Yun S, Zagmutt-Vergara FJ, Leutenegger CM, Bercovier H, Hedrick RP 2004: Concentrations of a Koi herpesvirus (KHV) in tissues of experimentally infected Cyprinus carpio koi as assessed by real-time TaqMan PCR. Dis Aquat Organ 60: 179-187 <https://doi.org/10.3354/dao060179>
6. Jonstrup SP, Kahns S, Skall HF, Boutrup TS, Olesen NJ 2013: Development and validation of a novel Taqman-based real-time RT-PCR assay suitable for demonstrating freedom from viral haemorrhagic septicaemia virus. J Fish Dis 36: 9-23 <https://doi.org/10.1111/j.1365-2761.2012.01416.x>
7. Lorenzen E, Carstensen B, Olesen NJ 1999: Inter-laboratory comparison of cell lines for susceptibility to three viruses: VHSV, IHNV and IPNV. Dis Aquat Org 37: 81-88 <https://doi.org/10.3354/dao037081>
8. Mackay IM 2004: Real-time PCR in the microbiology laboratory. Clin Microbiol Infect 10: 190-212 <https://doi.org/10.1111/j.1198-743X.2004.00722.x>
9. OIE Manual of Diagnostic Tests for Aquatic Animals 2016. Available at: http://www.oie.int/international-standard-setting/aquatic-manual/access-online/
10. Pierce LR, Stepien CA 2012: Evolution and biogeography of an emerging quasispecies: Diversity patterns of the fish Viral Hemorrhagic Septicemia virus (VHSv). Mol Phylogenet Evol 63: 327-341 <https://doi.org/10.1016/j.ympev.2011.12.024>
11. Reed LJ, Muench H 1938: A simple method of estimating fifty percent endpoints. Am J Epidemiol 27: 493-97 <https://doi.org/10.1093/oxfordjournals.aje.a118408>
12. Reichert M, Matras M, Skall HF, Olesen NJ, Kahns S 2013: Trade practices are main factors involved in the transmission of viral haemorrhagic septicaemia. J Fish Dis 36: 103-114 <https://doi.org/10.1111/jfd.12004>
13. Skall HF, Olesen NJ, Mellergaard S 2005: Viral haemorrhagic septicaemia virus in marine fish and its implications for fish farming – a review. J Fish Dis 28: 509-529 <https://doi.org/10.1111/j.1365-2761.2005.00654.x>
14. Snow M, Bain N, Black J, Taupin V, Cunningham CO, King JA. Skall HF, Raynard RS 2004: Genetic population structure of marine viral haemorrhagic septicaemia virus (VHSV). Dis Aquat Org 61: 11-21 <https://doi.org/10.3354/dao061011>
15. Spearman C 1906: The Proof and Measurement of Association between Two Things. Am J Psychol 15: 72-101 <https://doi.org/10.2307/1412159>
front cover
  • ISSN 0001-7213 (printed)
  • ISSN 1801-7576 (electronic)

Current issue

Indexed in DOAJ

Archive