Acta Vet. Brno 2017, 86: 317-323

https://doi.org/10.2754/avb201786040317

Designing a time-effective TaqMan probe-based real-time polymerase chain reaction protocol for the identification of Yersinia enterocolitica in raw pork meat

Milena Alicja Stachelska

Lomza State University of Applied Sciences, Institute of Food Technology and Gastronomy, Łomża, Poland

Received March 10, 2017
Accepted December 19, 2017

References

1. Dooley JJ, Paine KE, Garrett SD, Brown HM 2004: Detection of meat species using TaqMan real-time PCR assays. Meat Sci 68: 431-438 <https://doi.org/10.1016/j.meatsci.2004.04.010>
2. Girish PS, Anjaneyulu ASR, Viswas KN, Santhosh FH, Bhilegaonkar KN, Agarwal RK, Kondaiah N, Nagappa K 2007: Polymerase chain reaction–restriction fragment length polymorphism of mitochondrial 12S rRNA gene: a simple method for identification of poultry meat species. Vet Res Comm 31: 447-455 <https://doi.org/10.1007/s11259-006-3390-5>
3. Heptinstall J, Rapley R 2002: Spectrophotometric analysis of nucleic acids. In Rapley R (editor): The nucleic acid protocols handbook, pp 57–60, Totowa, N.J.: Humana Press
4. Herman L 1992: Determination of animal origin of raw food by species-species PCR. J Dairy Res 68: 429-436
5. Higgins DG, Bleasby AJ, Fuchs R 1992: CLUSTAL W: improved software for multiple sequence alignment. Comp Appl Biosci 8: 4420-4449
6. Hird H, Chisholm J, Brown J 2005: The detection of commercial duck species in food using a single probe-multiple species-specific primer real-time PCR assay. Eur Food Res Technol 221: 559-563 <https://doi.org/10.1007/s00217-005-1197-1>
7. Jonker KM, Tilburg JJHC, HaGele GH, De Boer E 2008: Species identification in meat products using real-time PCR. Food Add Contam 25: 527-533 <https://doi.org/10.1080/02652030701584041>
8. Kesmen Z, Sahin F, Yetim H 2007: PCR assay for the identification of animal species in cooked sausages. Meat Sci 77: 649-653 <https://doi.org/10.1016/j.meatsci.2007.05.018>
9. Kesmen Z, Gulluce A, Sahin F, Yetim H 2009: Identification of meat species by TaqMan-based real-time PCR assay. Meat Sci 82: 444-449 <https://doi.org/10.1016/j.meatsci.2009.02.019>
10. Kocher TD, Thomas WK, Meyer A, Edwards SV, Päabo S, Villablanca FX, Wilson AC 1989: Dynamics of mitochondrial DNA evolution in animals: amplification and sequencing with conserved primers. Proc Nat Acad Sci USA 86: 6196-6200 <https://doi.org/10.1073/pnas.86.16.6196>
11. Koppel R, Ruf J, Rentsch J 2011: Multiplex real-time PCR for the detection and quantification of DNA from beef, pork, horse and sheep. Eur Food Res Technol 232: 151-155 <https://doi.org/10.1007/s00217-010-1371-y>
12. Laube I, Zagon J, Spiegelberg A, Butschke A, Kroh LW, Broll H 2007: Development and design of a ‘ready-to-use’ reaction plate for a PCR-based simultaneous detection of animal species used in foods. Int J Food Sci Technol 42: 9-17 <https://doi.org/10.1111/j.1365-2621.2006.01154.x>
13. López-Andreo M, Garrido-Pertierra A, Puyet A 2006: Evaluation of postpolymerase chain reaction melting temperature analysis for meat species identification in mixed DNA samples. J Agric Food Chem 54: 7973-7978 <https://doi.org/10.1021/jf0615045>
14. Mane BG, Mendiratta SK, Tiwari AK 2009: Polymerase chain reaction assay for identification of chicken in meat and meat products. Food Chem 116: 806-810 <https://doi.org/10.1016/j.foodchem.2009.03.030>
15. Martín I, García T, Fajardo V, Rojas M, Pegels N, Hernández PE, Martín IG 2009: SYBR-Green real-time PCR approach for the detection and quantification of pig DNA in feedstuffs. Meat Sci 82: 252-259 <https://doi.org/10.1016/j.meatsci.2009.01.023>
16. Monteil-Sosa JF, Ruiz-Pesini E, Montoya J, Roncales P, López-Pérez MJ, Pérez-Martos A 2000: Direct and highly species-specific detection of pork meat and fat in meat products by PCR amplification of mitochondrial DNA. J Agric Food Chem 48: 2829-2832 <https://doi.org/10.1021/jf9907438>
17. Rodríguez MA, García T, González I, Asensio L, Mayoral B, López-Calleja I, Hernández PE, Martín R 2003: Identification of goose, mule duck, chicken, turkey, and swine in foie gras by species-specific polymerase chain reaction. J Agric Food Chem 51: 1524-1529 <https://doi.org/10.1021/jf025784+>
18. Rodriguez MA, García T, González I, Hernández PE, Martín R 2005: TaqMan real-time PCR for detection and quantitation of pork in meat mixtures. Meat Sci 70: 113-120 <https://doi.org/10.1016/j.meatsci.2004.12.005>
19. Saez R, Sanz Y, Toldrá F 2004: PCR-based fingerprinting techniques for rapid detection of animal species in meat products. Meat Sci 66: 659-665 <https://doi.org/10.1016/S0309-1740(03)00186-4>
20. Sawyer J, Wood C, Shanahan D, Gout S, McDowell D 2003: Real-time PCR for quantitative meat species testing. Food Cont 14: 579-583 <https://doi.org/10.1016/S0956-7135(02)00148-2>
21. Tanabe S, Hase M, Yano T, Sato M, Fujimura T, Akiyama H 2007. Real-time quantitative PCR detection method for pork, chicken, beef, mutton, and horse flesh in foods. Biosci Biotechnol Biochem 71: 3131-3135 <https://doi.org/10.1271/bbb.70683>
22. Walker JA, Hughes DA, Anders BA, Shewale J, Sinha SK, Batzer MA 2003: Quantitative intra-short interspersed element PCR for species-specific DNA identification. Anal Biochem 316: 259-269 <https://doi.org/10.1016/S0003-2697(03)00095-2>
front cover
  • ISSN 0001-7213 (printed)
  • ISSN 1801-7576 (electronic)

Current issue

Archive