Acta Vet. Brno 2017, 86: 333-338

The effect of Enterococcus faecium M74 feed additive on the extended-spectrum beta-lactamases/AmpC-positive Escherichia coli faecal counts in pre-weaned dairy calves

Jana Šmídková1,2, Alois Čížek1

1University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Medicine, Institute of Infectious Diseases and Microbiology, Brno, Czech Republic
2University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Medicine, Ruminant and Swine Clinic, Brno, Czech Republic

Received May 31, 2017
Accepted December 19, 2017


1. Berge AC, Atwill ER, Sischo WM 2005: Animal and farm influences on the dynamics of antibiotic resistance in faecal Escherichia coli in young dairy calves. Prev Vet Med 69: 25-38 <>
2. Busing K, Zeyner A 2014: Effects of oral Enterococcus faecium strain DSM 10663 NCIMB 10415 on diarrhea patterns and performance of sucking piglets. Benef Microbes 6: 41-44 <>
3. Carlet J 2012: The gut is the epicentre of antibiotic resistance. Antimicrob Resist In 1: 39 <>
4. Corbett EM, Norby B, Halbert LW, Henderson ST, Grooms DL, Manning SD, Kaneene JB 2015: Effect of feeding a direct-fed microbial on total and antimicrobial-resistant fecal coliform counts in preweaned dairy calves. Am J Vet Res 76: 780-788 <>
5. Gonggrijp MA, Santman-Berends IM, Heuvelink AE, Buter GJ, van Schaik G, Hage JJ, Lam TJ 2016: Prevalence and risk factors for extended-spectrum β-lactamase- and AmpC-producing Escherichia coli in dairy farms. J Dairy Sci 99: 9001-9013 <>
6. Geser N, Stephan R, Hächler H 2012: Occurrence and characteristics of extended-spectrum β- lactamase (ESBL) producing Enterobacteriaceae in food producing animals, minced meat and raw milk. BMC Vet Res 8: 21 <>
7. Hartmann A, Locatelli A, Amoureux L, Depret G, Jolivet C, Gueneau E, Neuwirth C 2012. Occurrence of CTX-M producing Escherichia coli in soils, cattle, and farm environment in france (Burgundy region). Front Microbiol 3: 83 <>
8. Hordijk J, Mevius DJ, Kant A, Bos ME, Graveland H, Bosman AB, Hartskeerl CM, Heederik DJJ, Wagenaar JA 2013: Within-farm dynamics of ESBL/AmpC-producing Escherichia coli in veal calves: a longitudinal approach. J Antimicrob Chemoth 68: 11
9. Hordijk J, Wagenaar JA, van de Giessen A, Dierikx C, van Essen-Zandbergen A, Veldeman K, Kant A, Mevius D 2013: Increasing prevalence and diversity of ESBL/AmpC-type-β-lactamase genes in Escherichia coli isolated from veal calves from 1997 to 2010. J Antimicrob Chemoth 68: 1970-1973 <>
10. Jamborova I, Dolejska M, Vojtech J, Guenther S, Uricariu R, Drozdowska J, Papousek I, Pasekova K, Meissner W, Hordowski J, Cizek A, Literak I 2015: Plasmid-mediated resistance to cephalosporins and fluoroquinolones in various Escherichia coli sequence types isolated from rooks wintering in Europe. Appl Environ Microbiol 81: 648-657 <>
11. Khachatryan AR, Hancock DD, Besser TE, Call DR 2004: Role of calf-adapted Escherichia coli in maintenance of antimicrobial drug resistance in dairy calves. Appl Environ Microbiol 70: 752-757 <>
12. Lema M, Williams L, Rao DR 2001: Reduction of fecal shedding of enterohemorrhagic Escherichia coli O157:H7 in lambs by feeding microbial feed supplement. Small Ruminant Res 39: 31-39 <>
13. Paivarinta M, Pohloja L, Fredriksson-Ahomaa M, Heikinheimo A 2016: Low cccurrence of extended-spectrum b-lactamase-producing Escherichia coli in Finnish food-producing animals. 2016 Blackwell Verlag GmbH, Zoonoses and Public Health 63: 624-631 <>
14. Pringsulaka O, Rueangyotchanthana K, Suwannasai N, Watanapokasin R, Amnueysit P, Sunthornthummas S, Sukkhum S, Sarawaneeyaruk S, Rangsiruji A 2015: In vitro sreening of lactic acid bacteria for multi-strain probiotics. Livest Sci 174: 66-73 <>
15. Pajarillo EAB, Chae JP, Balolong MP, Kim HB, Park CS, Kang DK 2015: Effects of probiotic Enterococcus faecium NCIMB 11181 administration on swine fecal microbiota diversity and composition using barcoded pyrosequencing. Anim Feed Sci Tech 201: 80-88 <>
16. Reeves HE, Lotz SB, Kennedy E, Randall LP, Coldham NG, La Ragione RM 2013: Evaluation of an autogenous vaccine in cattle against Escherichia coli bearing the CTX-M-14 plasmid. Res Vet Sci 94: 419-424 <>
17. Reg (EC) No 1831/2003. European Union Register of Feed Additives. Edition 250. Appendixes 3e, 4 – 24.01.2017 European Union legislation on feed additives. Available at:
18. Seiffert SN, Hilty M, Perreten V, Endimiani A 2013: Extended-spectrum cephalosporin-resistant gram-negative organisms in livestock: An emerging problem for human health? Drug Resist Update 16: 22-45 <>
19. Signorini ML, Soto LP, Zbrun MV, Sequeira GJ, Rosmini MR, Frizzo LS 2012: Impact of probiotic administration on the health and fecal microbiota of young calves: a meta-analysis of randomized controlled trials of lactic acid bacteria. Res Vet Sci 93: 250-258 <>
20. Snow LC, Warner RG, Cheney T, Wearing H, Stokes M, Harris K, Teale CJ, Coldham NG 2012: Risk factors associated with extended spectrum beta-lactamase Escherichia coli (CTX-M) on dairy farms in North West England and North Wales. Prev Vet Med 106: 225-234 <>
21. State Veterinary Administration 2017: National Program for Monitoring of Antimicrobial Resistance in Important Veterinary Pathogens. Newsletter 4: 23
22. Watson E, Jeckel S, Snow L, Stubbs R, Teale C, Wearing H, Horton R, Toszeghy M, Tearne O, Ellis-Iversen J, Coldham N 2012: Epidemiology of extended spectrum beta-lactamase E. coli (CTX-M-15) on a commercial dairy farm. Vet Microbiol 154 : 339-346 <>
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