Acta Vet. Brno 2017, 86: 399-404
Improvement of colitis by Lactobacillus plantarum LS/07 and inulin
The objective of this study was to investigate the effect of the probiotic strain Lactobacillus plantarum LS/07 and prebiotic inulin on the activity of β-glucuronidase, transcription nuclear factor kappa B (NFκB), myeloperoxidase (MPO), cytokine levels (IL-6, IL-8, IL-13), and counts of coliforms and lactobacilli in rats with dextran sulphate sodium (DSS) induced acute colitis. Sprague-Dawley rats were divided into groups: control (C), acute colitis (AC), prebiotic (Pre), and probiotic (Pro). Dextran sulphate sodium induced inflammatory process in the colonic tissue, increased the activity of β-glucuronidase (P < 0.05), increased counts of coliforms, decreased lactobacilli counts (P < 0.05), and activated production of the measured indicators NFκB, MPO, IL-6, IL-8, except of IL-13. Diet supplemented with L. plantarum and inulin alleviated DSS induced inflammatory process by inhibiting production of IL-6, IL-8, activities of NFκB and MPO, and by stimulation of IL-13. These results indicate that the dietary intake of Lactobacillus plantarum LS/07 and inulin suppressed the expression of markers playing an important role in the inflammatory process, which predisposes their use in prevention or treatment of acute colitis.
Keywords
Inflammatory bowel diseases, rats, inflammation, prebiotics, probiotics.
Funding
This work was supported by the Research Agency Slovak Republic for the Structural Funds of the EU under project ITMS 26220120058, ITMS 26220220104 and grant 4/GSD/2012.
