Acta Vet. Brno 2018, 87: 47-54

Simultaneous determination of β-agonists and monitoring in bovine tissues by liquid chromatography-tandem mass spectrometry

Kyunghun Jeong1, Akil Hossain1, Hae-chul Park1, Seong-Wan Son2, JeongWoo Kang1

1Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency (APQA), Gimcheon 177, Republic of Korea
2Ministry of Food and Drug Safety, Osong Health Technology Administration Complex, Cheongju, Republic of Korea

Received January 31, 2017
Accepted April 3, 2018


1. Anderson DB, Moody DE, Hancock DL 2009: Beta adrenergic agonists. Marcel Dekker Inc., New York, pp 104-107
2. Angeletti R, Oriundi MP, Piro R, Bagnati R 1993: Application of an enzyme-linked immunosorbent assay kit for β-agonist screening of bovine urines in north-eastern Italy. Anal Chim Acta 275: 215-219 <>
3. Antignac JP, Marchand P, Le Bizec B, Andre F 2002: Identification of ractopamine residues in tissue and urine samples at ultra-trace level using liquid chromatography–positive electrospray tandem mass spectrometry. J Chromatogr B 774: 59-66 <>
4. Association of Analytical Chemists (AOAC): Standard Format and Guidance for AOAC Standard Method Performance Requirement. Available from: Last modified January 31, 2011. Accessed January 25, 2017
5. Batjoens P, Courtheyn D, De Brabander HF, Vercammen J, Wasch KD, Logghe M 1996: Gas chromatographic-tandem mass spectrometric analysis of clenbuterol residues in feces. J Chromatogr A 750: 133-139 <>
6. Blomgren A, Berggren C, Holmberg A, Larsson F, Sellergren B, Ensing K 2002: Extraction of clenbuterol from calf urine using a molecularly imprinted polymer followed by quantitation by high-performance liquid chromatography with UV detection. J Chromatogr A 975: 157-164 <>
7. Bocca B, Di Mattia M, Cartoni C, Fiori M, Felli M, Neri B, Brambilla G 2003: Extraction, clean-up and gas chromatography–mass spectrometry characterization of zilpaterol as feed additive in fattening cattle. J Chromatogr B783: 141-149 <>
8. Codex Alimentarius Commission: Maximum Residue Limits for Veterinary Drugs in Foods: Updated as at the 38th session of the Codex Alimentarius Commission. Available from:,d.bGg. Last modified July 23, 2015. Accessed January 25, 2017
9. Degand G, Bernes-Duyckaerts A, Delahaut P, Maghuin-Rogister G 1993: Determination of β-agonists in urine by enzyme immunoassay based on the use of an anti-salbutamol antiserum. Anal Chim Acta 275: 241-247 <>
10. European Commission: Concerning the Performance of Analytical Methods and the Interpretation of Results. Available from: Last modified august 17, 2002. Accessed January 25, 2017
11. European Commission: On Measures to Monitor Certain Substances and Residues Thereof in Live Animals and Animal Products and Repealing. Available at: Last modified May 23, 1996. Accessed January 25, 2017
12. Hossain MA, Park HC, Jeong KH, Jang YH, Kim DG, Kang JW, Lee KJ 2017: Pharmacokinetic and pharmacodynamic evaluation of marbofloxacin in pig against Korean local isolates of Actinobacillus pleuropneumoniae. BioMed Res Int 2017: 1-11
13. International Conference on Harmonization (ICH): Validation of Analytical Procedures: Methodology of Technical Requirements for Registration of Pharmaceuticals for Human Use. Available from: Last modified November 29, 1996. Accessed January 25, 2017
14. Kulikovskii AV, Lisitsyn AB, Gorlov IF, Slozhenkina MI, Savchuk SA 2016: Determination of growth hormones (β-agonists) in muscle tissue by HPLC with mass spectrometric detection. J Anal Chem 71: 1052-1056 <>
15. Liu B, Yan H, Qiao F, Geng Y 2011: Determination of clenbuterol in porcine tissues using solid-phase extraction combined with ultrasound-assisted dispersive liquid–liquid microextraction and HPLC–UV detection. J Chromatogr B 879: 90-94 <>
16. Martinez-Navarro JF 1990: Food poisoning related to consumption of illicit β-agonist in liver. Lancet 336: 1311 <>
17. Morales-Trejo F, León SVY, Escobar-Medina A, Gutiérrez-Tolentino R 2013: Application of high-performance liquid chromatography–UV detection to quantification of clenbuterol in bovine liver samples. J Food Drug Anal 21: 414-420 <>
18. OMCL Network/EDQM of the Council of Europe: Validation of Analytical Procedures PA/PH/OMCL (05) 47 DEF. Available from: Last modified June 15, 2005. Accessed January 25, 2017
19. Wang L, Zeng Z, Wang X, Yang J, Chen Z, He L 2013: Multiresidue analysis of nine β‐agonists in animal muscles by LC‐MS/MS based on a new polymer cartridge for sample cleanup. J Sep Sci 36: 1843-1852 <>
20. Yan K, Zhang H, Hui W, Zhu H, Li X, Zhong F, Chen C 2016: Rapid screening of toxic salbutamol, ractopamine, and clenbuterol in pork sample by high performance liquid chromatography-UV method. J Food Drug Anal 24: 277-283 <>
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  • ISSN 1801-7576 (electronic)

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