Acta Vet. Brno 2018, 87: 55-64

Oxidative stress response of rainbow trout (Oncorhynchus mykiss) to multiple stressors

Radovan Kopp1, Miroslava Palíková2, Ivana Papežíková2, Jan Mareš1, Stanislav Navrátil2, Jiří Pikula2, Miroslav Pohanka3

1Mendel University in Brno, Faculty of AgriSciences, Department of Fisheries and Hydrobiology, Brno, Czech Republic
2University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Hygiene and Ecology, Department of Ecology and Diseases of Game, Fish and Bees, Brno, Czech Republic
3University of Defence, Faculty of Military Health Sciences, Centre of Advanced Studies, Hradec Kralove, Czech Republic

Received May 3, 2017
Accepted April 3, 2018

Fish can be exposed to multiple stressors at the same time, including natural toxins, environmental pollutants or feed contamination. In this study, we report on the effects of controlled exposure of rainbow trout (Oncorhynchus mykiss) to multiple stressors. In doing so, we test the hypothesis that cyanobacterial biomass and arsenic in feed combine to enhance negative effects on fish, including modulation of antioxidant response. The trout (mean weight 288 ± 59 g) were divided into six experimental groups of 25 fish each (C - control group; B - 3% lyophilised toxic cyanobacterial biomass; As5 - arsenic at 5 mg·kg–1; As50 - arsenic at 50 mg·kg–1; and two combinations of cyanobacterial biomass and arsenic (B+As5 and B+As50). All indicators of oxidative stress were determined using standard methods, showing significant changes. Overall antioxidant capacity (ferric reducing antioxidant power) decreased significantly in the liver, gills and heart after 10-days, while lipid peroxidation (thiobarbituric acid reactive substances) increased in the liver of groups exposed to cyanobacterial biomass, and in the heart of groups exposed to increased concentrations of arsenic and those under combined exposure. Levels of glutathione reductase showed a significant reduction following exposure, especially in the gills and liver. Our results indicate that the antioxidant system plays an important role in the protective response of tissues to stressors, and especially arsenic.


Fish, arsenic, microcystin, FRAP, TBARS, GR.


This work was supported by a research project of the National Agency for Agricultural Research of the Czech Republic (QJ1510077) and by IGA VFU Brno (project 220/2016/FVHE).


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