Acta Vet. Brno 2018, 87: 387-393

Antibacterial effect of Czech and Mānuka honey on selected mastitis pathogens

Marcela Klimešová, Irena Němečková, Eva Vondrušková, Ludmila Nejeschlebová

Dairy Research Institute, Prague, Czech Republic

Received May 28, 2018
Accepted December 7, 2018

The work is focused on the antibacterial effect of four types of blossom honey, one honeydew and two Mānuka honey (MAN100+ and MAN400+) on selected pathogenic microorganisms isolated from cow’s milk (Staphylococcus aureus 51 and S. aureus 428), sheep’s milk (S. aureus 627), and from the Czech Collection of Microorganisms (Streptococcus uberis CCM 4617, Streptococcus agalactiae CCM 6187, Enterococcus faecalis CCM 4224 and Escherichia coli CCM 4787). The concentrations of honey samples were 20% and 30%. The obtained results showed a 100% inhibitory effect of MAN400+ on all tested bacterial strains even at a concentration of 20% and also a comparable inhibitory effect of Mānuka honey with Czech honeydew. The results indicate that honey had an inhibitory effect against the tested bacterial species which may cause mastitis.


This work was supported by Ministry of Agriculture of Czech Republic by the project NAZV KUS QJ1510047 and QJ1510339 and decision No. RO1418


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