Acta Vet. Brno 2019, 88: 103-111

PCR-RFLP identification of meat from red deer, sika deer, roe deer, fallow deer, mouflon, wild boar, hare and cattle

Michaela Nesvadbová1,2, Gabriela Bořilová1,2, Radka Hulánková1,2

1CEITEC - Central European Institute of Technology, Brno, Czech Republic
2University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Hygiene and Ecology, Department of Meat Hygiene and Technology, Brno, Czech Republic

Received October 24, 2018
Accepted February 12, 2019


1. Abdigini A, Zibibilla G, Abduriyim S, Halic M 2015: Species identification of unknown animals based on mt DNA 16S rRNA gene sequencing. Chinese J of Forens Med 1: 008
2. Act No 110/1997 Collection of Laws of the Czech Republic, on food and tobacco products and on the amendment and supplementation of some related acts. Available at:–110. Accessed: October 22, 2018
3. Act No 166/1999 Collection of Laws of the Czech Republic, on veterinary care and on on the amendment of some related acts (the Veterinary Act). Available at:–166. Accessed: October 22, 2018
4. Alikord M, Momtaz H, Kadivar M, Rad AH 2018: Species identification and animal authentication in meat products: a review. J Food Meas Charact 12: 145-155 <>
5. Amaral JS, Santos CG, Melo VS, Oliveira MP, Mafra I 2014: Authentication of a traditional game meat sausage (Alheira) by species-specific PCR assays to detect hare, rabbit, red deer, pork and cow meats. Food Res International 60: 140-145 <>
6. Amaral JS, Santos CG, Melo VS, Costa J, Oliveira MBP, Mafra I 2015: Identification of duck, partridge, pheasant, quail, chicken and turkey meats by species-specific PCR assays to assess the authenticity of traditional game meat Alheira sausages. Food Control 47: 190-195 <>
7. Barbuto M, Galimberti A, Ferri E, Labra M, Malandra R, Galli P, Casiraghi M 2010: DNA barcoding reveals fraudulent substitutions in shark seafood products: The Italian case of “palombo” (Mustelus spp.). Food Res International 43: 376-381 <>
8. Druml B, Grandits S, Mayer W, Hochegger R, Cichna-Markl M 2015: Authenticity control of game meat products–A single method to detect and quantify adulteration of fallow deer (Dama dama), red deer (Cervus elaphus) and sika deer (Cervus nippon) by real-time PCR. Food Chem 170: 508-517 <>
9. El-Jaafari HA, Panandam JM, Idris I, Sriaj SS 2008: RAPD analysis of three deer species in Malaysia. Asian Austral J Anim, 21: 1233-1237 <>
10. Fajardo V, González I, López-Calleja I, Martín I, Hernández P E, García T, Martín R 2006: PCR-RFLP authentication of meats from red deer (Cervus elaphus), fallow deer (Dama dama), roe deer (Capreolus capreolus), cattle (Bos taurus), sheep (Ovis aries), and goat (Capra hircus). J Agri Food Chem 54: 1144-1150 <>
11. Fajardo V, González I, Rojas M, García T, Martín R 2010: A review of current PCR-based methodologies for the authentication of meats from game animal species. Trends Food Sci Tech 21: 408-421 <>
12. Farag MR, Alagawany M, Abd El-Hack ME, Tiwari R, Dhama K 2015: Identification of different animal species in meat and meat products: trends and advances. Adv Anim Vet Sci 3: 334-346 <>
13. Guan F, Jin YT, Zhao J, Xu AC, Luo YY 2018: A PCR Method that can be further eveloped into PCR-RFLP assay for Eeight animal species identification. J Anal Methods Chem, Vol 2018, Article ID 5890140 <>
14. Gupta AR, Patra RC, Das DK, Gupta PK, Swarup D, Saini M 2008: Sequence characterization and polymerase chain reaction-restriction fragment length polymorphism of the mitochondrial DNA 12S rRNA gene provides a method for species identification of Indian deer: Mitochondr DNA 19: 394-400
15. Ha JC, Jung WT, NamYS, Moon TW 2006: PCR identification of ruminant tissue in raw and heat-treated meat meals. J Food Prot 69: 2241-2247 <>
16. Hossain MM, Ali ME, Hamid SBA, Mustafa S, Desa MNM, Zaidul ISM 2017: Targeting double genes in multiplex PCR for discriminating bovine, buffalo and porcine materials in food chain. Food Control 73: 175-184 <>
17. Jiang C, Jin Y, Zhao X, Yuan Y, Zhao Y, Huang L 2018: Rapid and robust authentication of deer antler velvet product by fast PCR-RFLP analysis. Mitochondr DNA Part A 29: 266-272 <>
18. Kaltenbrunner M, Hochegger R, Cichna-Markl M 2018: Tetraplex real-time PCR assay for the simultaneous identification and quantification of roe deer, red deer, fallow deer and sika deer for deer meat authentication. Food Chem 269: 486-494 <>
19. Kumar D, Singh SP, Karabasanavar NS, Singh R, Umapathi V 2014: Authentication of beef, carabeef, chevon, mutton and pork by a PCR-RFLP assay of mitochondrial cytb gene. J Food Sci Tech 51: 3458-3463 <>
20. Lo YT, Shaw PC 2018: DNA-based techniques for authentication of processed food and food supplements. Food Chem 240: 767-774 <>
21. Lockley AK, Bardsley RG 2000: DNA-based methods for food authentication. Trends Food Sci Tech 11: 67-77 <>
22. Martinez I, Yman IM 1998: Species identification in meat products by RAPD analysis. Food Res International 31: 459-466 <>
23. Partis L, Croan D, Guo Z, Clark R, Coldham T, Murby J 2000: Evaluation of a DNA fingerprinting method for determining the species origin of meats. Meat Sci 54: 369-376 <>
24. Pfeiffer I, Burger J, Brenig B 2004: Diagnostic polymorphisms in the mitochondrial cytochrome b gene allow discrimination between cattle, sheep, goat, roe buck and deer by PCR-RFLP. BMC Genet 5: 30 <>
25. Regulation (EC) No 172/2002 of the European Parliament and of the Council of 28 January 2002 laying down the general principles and requirements of food law, establishing the European Food Safety Authority and laying down procedures in matters of food safety. Available at: Accessed: October 22, 2018
26. Regulation (EC) No 1169/2011of the European Parliament and of the Council of 25 October 2011 on the provision of food information to consumers. Available at: Accessed: October 22, 2018
27. Şakalar E, Abasiyanik MF, Bektik E, Tayyrov A 2012: Effect of heat processing on DNA quantification of meat species. J Food Sci 77: N40-N44 <>
28. Santos CG, Melo VS, Amaral JS, Estevinho L, Oliveira MBPP, Mafra I 2012: Identification of hare meat by a species-specific marker of mitochondrial origin. Meat Sci 90: 836-841 <>
29. Yang L, Tan Z, Wang D, Xue L, Guan MX, Huang T, Li R 2014: Species identification through mitochondrial rRNA genetic analysis. Sci Rep-Uk 4: 4089 <>
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  • ISSN 0001-7213 (printed)
  • ISSN 1801-7576 (electronic)

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