Acta Vet. Brno 2019, 88: 129-141

The evaluation of glutathione concentration in whole blood of Holstein dairy calves

Andrea Nečasová1, Alena Pechová1, R. Bodor2, M. Masár2, Milada Holasová1

1University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Hygiene and Ecology, Department of Animal Protection, Welfare and Behaviour, Brno, Czech Republic
2Comenius University Bratislava, Faculty of Natural Sciences, Department of Analytical Chemistry, Bratislava, Slovakia

Received February 4, 2019
Accepted April 23, 2019

The aim of this study was to evaluate the concentrations of total (T-GSH), oxidized (GSSG) and reduced glutathione (GSH) in blood of clinically healthy calves, to assess the effect of sex and age together with the type of feeding on its concentrations, and to assess the relationships between glutathione and metabolic indices. A total of 117 Holstein calves at the age of 1 day to 6 months were divided according to age and together with the type of feeding into 4 groups: colostrum and transition milk (n = 20), native milk (n = 39), milk replacer (n = 29) and total mixed ration (n = 29). Blood serum was used for the assessment of the metabolic profile of calves. The concentrations of individual forms of glutathione (mean ± SEM) were as follows: T-GSH: 789.1 ± 19.4 µmol/l, GSSG: 54.2 ± 4.3 µmol/l, GSH: 681.6 ± 15.3 µmol/l, and GSH/GSSG: 41.1 ± 5.9, with the coefficients of variability of 26.6%, 85.3%, 24.3% and 157.4%, respectively. Age and the type of feeding affected GSSG (P ≤ 0.01) and GSH/GSSG (P ≤ 0.01). The relationships between glutathione and biochemical parameters were mostly weak. T-GSH correlated with creatinine (P ≤ 0.05) and chlorides (P ≤ 0.05); GSSG correlated with albumin (P ≤ 0.01), creatinine (P ≤ 0.01), cholesterol (P ≤ 0.05), aspartate aminotransferase (P ≤ 0.01), alanine aminotransferase (P ≤ 0.01), gamma-glutamyltransferase (P ≤ 0.05) and calcium (P ≤ 0.05); GSH/GSSG ratio correlated with creatinine (P ≤ 0.01), cholesterol (P ≤ 0.05), aspartate aminotransferase (P ≤ 0.05), gamma-glutamyltransferase (P ≤ 0.01) and calcium (P ≤ 0.05). The evaluation of individual forms of glutathione helps in estimation of redox status in healthy animals and their monitoring can detect the stress extent.


24 live references