Acta Vet. Brno 2019, 88: 165-168

Detection of Fusobacterium necrophorum and Dichelobacter nodosus from cow footrot in the Heilongjiang Province, China

Jiaqi Han, Jing Dong, Wenhui Yang, Yunze Xu, Qiming Xing, Yuelei Chen, Lin Li

Shenyang Agricultural University, College of Animal Science & Veterinary Medicine, The Key Laboratory of Zoonosis of Liaoning Province, Shenyang, China

Received February 13, 2018
Accepted April 23, 2019

Cow footrot in the Heilongjiang Province, northeast China is a problem resulting in lost production in agriculture. In this study, 200 swab samples from footrot lesions of naturally infected cows with odorous exudative inflammation and keratinous hoof separation at 10 farms were examined in the period from May 2016 to May 2017. Twenty cows from each farm were taken for sampling. The samples were examined for detectingthe presence of Dichelobacter nodosus (D. nodosus) and Fusobacterium necrophorum (F. necrophorum). Such detection was carried out using polymerase chain reaction (PCR). The PCR primers were designed to identify the lktA gene, which encodes a leukotoxin unique to F. necrophorum, and the fimA gene of D. nodosus. Of the 200 samples, 111 (55.5%) revealed the presence of F. necrophorum and 11 (5.5%) exhibited D. nodosus. The frequent finding of F. necrophorum in cow farms of Heilongjiang province, northeast China is noteworthy. The possibility of F. necrophorum and D. nodosus infection should be an important concern when controlling cow footrot in China.


This study was supported by the National Key Research and Development Program of China (Project No. 2017YFD0502200).


22 live references